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Literature summary for 3.1.1.20 extracted from

  • Kar, B.; Banerjee, R.; Bhattacharyya, B.C.
    Effect of additives on the behavioural properties of tannin acyl hydrolase (2003), Proc. Biochem., 38, 1285-1293.
No PubMed abstract available
Search for more literature for 3.1.1.20

Activating Compound

Activating Compound Comment Organism Structure
ammonium chloride 0.1% w/v, 4fold increased activity when used as nitrogen source Rhizopus arrhizus
ammonium nitrate 0.1% w/v, 1.2fold increased activity when used as nitrogen source Rhizopus arrhizus
ammonium sulfate with FeSO4, when used as nitrogen source, 1.7fold increase in activity Rhizopus arrhizus
Br- 1.6fold induction Rhizopus arrhizus
Hg+ slight activation at 1 mM, 105% activity Rhizopus arrhizus
Mg2+ activation at 1 mM, 137.9% activity Rhizopus arrhizus
S2O32- 2.2fold induction Rhizopus arrhizus
Tween 40 best at 0.4% v/v, above that concentration it inhibits the enzyme Rhizopus arrhizus
Tween 80 best at 0.05% v/v, above that concentration it inhibits the enzyme Rhizopus arrhizus
Urea at 1.5 M maximal enhancement of activity, at 3 M 50% inhibition Rhizopus arrhizus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline slight inhibition, 1 mM, 88% remaining activity Rhizopus arrhizus
2-mercaptoethanol 1 mM, 68.3% remaining activity Rhizopus arrhizus
Ag+ slight competitive inhibition at 1 mM, 82.4% remaining activity Rhizopus arrhizus
Ba2+ competitive inhibition at 1 mM, 5.4% remaining activity Rhizopus arrhizus
Ca2+ slight competitive inhibition at 1 mM, 86.6% remaining activity Rhizopus arrhizus
Co2+ complete inhibition at 1 mM Rhizopus arrhizus
CO32-
-
 Rhizopus arrhizus
DMSO 1 mM, 19% remaining activity Rhizopus arrhizus
EDTA 1 mM, 30% remaining activity Rhizopus arrhizus
Fe3+ complete inhibition at 1 mM Rhizopus arrhizus
Hg2+ competitive inhibition at 1 mM, 13.8% remaining activity Rhizopus arrhizus
OH-
-
 Rhizopus arrhizus
S2O82-
-
 Rhizopus arrhizus
sodium lauryl sulfate
-
 Rhizopus arrhizus
soybean extract inhibits at 0.05-1.0% w/v Rhizopus arrhizus
Triton X-100
-
 Rhizopus arrhizus
Tween 40 activation up to 0.05% v/v, complete inhibition at 0.6% v/v; activation up to 0.4% v/v, complete inhibition at 0.6% v/v Rhizopus arrhizus
Tween 60 complete inhibition at 1% v/v Rhizopus arrhizus
Urea at 1.5 M maximal enhancement of activity, at 3 M 50% inhibition Rhizopus arrhizus
Zn2+ competitive inhibition at 1 mM, 21.3% remaining activity Rhizopus arrhizus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhizopus arrhizus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + 10 H2O Rhizopus arrhizus
-
 10 gallic acid + glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tannic acid + 10 H2O
-
 Rhizopus arrhizus 10 gallic acid + glucose
-
 ?

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
 Rhizopus arrhizus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Rhizopus arrhizus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.49
-
 Ba2+ 40°C Rhizopus arrhizus
0.5
-
 Ag+ 40°C Rhizopus arrhizus
0.57
-
 Hg2+ 40°C Rhizopus arrhizus
8.25
-
 Zn2+ 40°C Rhizopus arrhizus