KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
Tannic acid | free enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C | Aspergillus niger | |
0.433 | - |
methyl gallate | free enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C | Aspergillus niger | |
0.529 | - |
methyl gallate | Ca alginate-immobilized enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C | Aspergillus niger | |
23.75 | - |
Tannic acid | Ca alginate-immobilized enzyme, in citrate buffer (50 mM, pH 5.5), at 30°C | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger GH1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by HiTrap G25 gel filtration (5.4fold purification and 5.6fold concentration with a recovery yield higher than 90%) | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | - |
Aspergillus niger | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Aspergillus niger GH1 | gallate + methanol | - |
? | |
tannic acid + H2O | - |
Aspergillus niger | gallic acid + D-glucose | - |
? | |
tannic acid + H2O | - |
Aspergillus niger GH1 | gallic acid + D-glucose | - |
? |