KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
methyl gallate | pH and temperature not specified in the publication | Enterobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, SDS-PAGE | Enterobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Enterobacter sp. | - |
gallic acid + D-glucose | - |
? | |
tannic acid + H2O | Enterobacter sp. KPJ03 | - |
gallic acid + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. | C7EDT0 | - |
- |
Enterobacter sp. KPJ03 | C7EDT0 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-100 gel filtration | Enterobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.084 | - |
crude extract, pH and temperature not specified in the publication | Enterobacter sp. |
13.63 | - |
after 162fold purification, pH and temperature not specified in the publication | Enterobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | - |
Enterobacter sp. | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Enterobacter sp. KPJ03 | gallate + methanol | - |
? | |
tannic acid + H2O | - |
Enterobacter sp. | gallic acid + D-glucose | - |
? | |
tannic acid + H2O | - |
Enterobacter sp. KPJ03 | gallic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Enterobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
tannin acyl hydrolase | - |
Enterobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Enterobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme retains 20% of its activity at 60°C | Enterobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Enterobacter sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | tannase and the organism itself are employed to protect grazing animals and environment against the toxic effects caused by tannins in them | Enterobacter sp. |