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Literature summary for 3.1.1.20 extracted from
- Biosynthesis, structural architecture and biotechnological potential of bacterial tannase: a molecular advancement (2014), Biores. Technol., 157, 327-340.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Lactiplantibacillus plantarum |  |
| SDS | 1% (v/v) SDS shows stimulatory (13.2%) effect on tannase activity | Bacillus subtilis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bacillus subtilis | |
| 2-mercaptoethanol | - |
Lactiplantibacillus plantarum | |
| Ag+ | - |
Bacillus subtilis | |
| Al3+ | - |
Escherichia coli | |
| Ba2+ | - |
Bacillus subtilis | |
| Ba2+ | - |
Enterobacter cloacae | |
| Ca2+ | - |
Bacillus subtilis | |
| Co2+ | - |
Bacillus subtilis | |
| Co2+ | - |
Rhodococcus sp. | |
| Cu2+ | - |
Bacillus subtilis | |
| Cu2+ | - |
Enterobacter cloacae | |
| Cu2+ | - |
Escherichia coli | |
| Cu2+ | - |
Rhodococcus sp. | |
| DMSO | most potent inhibitor | Bacillus subtilis | |
| DMSO | - |
Lactiplantibacillus plantarum | |
| EDTA | - |
Escherichia coli | |
| Fe2+ | - |
Bacillus subtilis | |
| Fe2+ | - |
Enterobacter cloacae | |
| Fe3+ | - |
Bacillus subtilis | |
| Fe3+ | - |
Escherichia coli | |
| Fe3+ | - |
Rhodococcus sp. | |
| Hg2+ | - |
Bacillus subtilis | |
| Hg2+ | - |
Lactiplantibacillus plantarum | |
| Hg2+ | - |
Rhodococcus sp. | |
| K+ | - |
Bacillus subtilis | |
| methyl gallate | - |
Bacillus subtilis | |
| methyl gallate | - |
Enterobacter cloacae | |
| Mg2+ | - |
Lactiplantibacillus plantarum | |
| propyl gallate | - |
Bacillus subtilis | |
| propyl gallate | - |
Enterobacter cloacae | |
| pyrogallol | - |
Bacillus subtilis | |
| pyrogallol | - |
Enterobacter cloacae | |
| SDS | - |
Enterobacter cloacae | |
| Sodium azide | - |
Bacillus subtilis | |
| Sodium azide | - |
Enterobacter cloacae | |
| Sodium thioglycolate | - |
Bacillus subtilis | |
| Sodium thioglycolate | - |
Enterobacter cloacae | |
| Triton X-100 | - |
Bacillus subtilis | |
| Triton X-100 | - |
Enterobacter cloacae | |
| Triton X-100 | - |
Lactiplantibacillus plantarum | |
| Tween 20 | - |
Enterobacter cloacae | |
| Tween 60 | - |
Bacillus subtilis | |
| Tween 80 | - |
Bacillus subtilis | |
| Tween 80 | - |
Enterobacter cloacae | |
| Tween 80 | - |
Lactiplantibacillus plantarum | |
| Urea | - |
Lactiplantibacillus plantarum | |
| Zn2+ | - |
Bacillus subtilis | |
| Zn2+ | - |
Escherichia coli | |
| Zn2+ | - |
Lactiplantibacillus plantarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00337 | - |
Tannic acid | pH and temperature not specified in the publication | Enterobacter cloacae | |
| 0.445 | - |
Tannic acid | pH and temperature not specified in the publication | Bacillus subtilis | |
| 1.6 | - |
methyl gallate | pH and temperature not specified in the publication | Selenomonas ruminantium | |
| 3.7 | - |
methyl gallate | pH and temperature not specified in the publication | Enterobacter sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Lactiplantibacillus plantarum | |
| K+ | - |
Lactiplantibacillus plantarum | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Bacillus subtilis | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Escherichia coli | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Enterobacter cloacae | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Lysinibacillus sphaericus | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Bacillus cereus | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Klebsiella pneumoniae | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Selenomonas ruminantium | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Rhodococcus sp. | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Enterobacter sp. | |
| Mg2+ | Mg2+ is the most common requirement for optimum activity | Serratia ficaria | |
| Mn2+ | - |
Escherichia coli | |
| Mn2+ | - |
Enterobacter cloacae | |
| NH4+ | - |
Escherichia coli | |
| Ni2+ | - |
Escherichia coli | |
| Zn2+ | - |
Enterobacter cloacae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Enterobacter cloacae |
| 46500 | - |
x * 46500, SDS-PAGE | Klebsiella pneumoniae |
| 50700 | - |
x * 50700, SDS-PAGE | Lactiplantibacillus plantarum |
| 52000 | - |
x * 52000 | Bacillus subtilis |
| 55000 | - |
x * 55000, SDS-PAGE | Escherichia coli |
| 59000 | - |
x * 59000, SDS-PAGE | Selenomonas ruminantium |
| 60000 | - |
x * 60000, SDS-PAGE | Rhodococcus sp. |
| 90000 | - |
x * 90000, SDS-PAGE | Enterobacter sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | Lactiplantibacillus plantarum | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Bacillus subtilis | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Escherichia coli | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Enterobacter cloacae | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Lysinibacillus sphaericus | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Bacillus cereus | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Klebsiella pneumoniae | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Selenomonas ruminantium | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Rhodococcus sp. | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Enterobacter sp. | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Serratia ficaria | - |
gallate + D-glucose | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | the activity of tannase increases in presence of ethanol | Bacillus subtilis |
| Glycerol | the activity of tannase increases in presence of glycerol | Bacillus subtilis |
| isoamyl alcohol | the activity of tannase increases in presence of isoamyl alcohol | Bacillus subtilis |
| isopropanol | the activity of tannase increases in presence of isopropanol | Bacillus subtilis |
| Methanol | the activity of tannase increases in presence of methanol | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Enterobacter cloacae | - |
- |
- |
| Enterobacter sp. | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
| Lactiplantibacillus plantarum | - |
- |
- |
| Lysinibacillus sphaericus | - |
- |
- |
| Rhodococcus sp. | - |
- |
- |
| Selenomonas ruminantium | - |
- |
- |
| Serratia ficaria | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation followed by Sephadex G-75 gel filtration | Bacillus subtilis |
| ammonium sulfate precipitation, Q-Sepharose column chromatography, hydroxylapatite column chromatography, and MonoQ column chromatography | Lactiplantibacillus plantarum |
| DEAE-cellulose chromatography followed by Sephadex G-100 gel filtration | Enterobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Bacillus subtilis | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Escherichia coli | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Enterobacter cloacae | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Lysinibacillus sphaericus | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Bacillus cereus | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Klebsiella pneumoniae | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Selenomonas ruminantium | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Rhodococcus sp. | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Enterobacter sp. | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Serratia ficaria | gallate + methanol | - |
? | |
| tannic acid + H2O | - |
Lactiplantibacillus plantarum | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Bacillus subtilis | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Escherichia coli | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Enterobacter cloacae | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Lysinibacillus sphaericus | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Bacillus cereus | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Klebsiella pneumoniae | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Selenomonas ruminantium | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Rhodococcus sp. | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Enterobacter sp. | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Serratia ficaria | gallate + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, SDS-PAGE | Enterobacter sp. |
| ? | x * 60000, SDS-PAGE | Rhodococcus sp. |
| ? | x * 55000, SDS-PAGE | Escherichia coli |
| ? | x * 59000, SDS-PAGE | Selenomonas ruminantium |
| ? | x * 31000, SDS-PAGE | Enterobacter cloacae |
| ? | x * 46500, SDS-PAGE | Klebsiella pneumoniae |
| ? | x * 52000 | Bacillus subtilis |
| ? | x * 50700, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tannin acyl hydrolase | - |
Lactiplantibacillus plantarum |
| tannin acyl hydrolase | - |
Bacillus subtilis |
| tannin acyl hydrolase | - |
Escherichia coli |
| tannin acyl hydrolase | - |
Enterobacter cloacae |
| tannin acyl hydrolase | - |
Lysinibacillus sphaericus |
| tannin acyl hydrolase | - |
Bacillus cereus |
| tannin acyl hydrolase | - |
Klebsiella pneumoniae |
| tannin acyl hydrolase | - |
Selenomonas ruminantium |
| tannin acyl hydrolase | - |
Rhodococcus sp. |
| tannin acyl hydrolase | - |
Enterobacter sp. |
| tannin acyl hydrolase | - |
Serratia ficaria |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Rhodococcus sp. |
| 30 | 40 | - |
Lactiplantibacillus plantarum |
| 30 | 40 | - |
Selenomonas ruminantium |
| 35 | - |
- |
Escherichia coli |
| 35 | - |
- |
Serratia ficaria |
| 40 | - |
- |
Bacillus subtilis |
| 40 | - |
- |
Bacillus cereus |
| 40 | - |
- |
Klebsiella pneumoniae |
| 40 | - |
- |
Enterobacter sp. |
| 50 | - |
- |
Enterobacter cloacae |
| 50 | - |
- |
Lysinibacillus sphaericus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | - |
Escherichia coli |
| 30 | 70 | - |
Bacillus subtilis |
| 30 | 80 | - |
Lysinibacillus sphaericus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Bacillus cereus |
| 5 | - |
- |
Bacillus subtilis |
| 5 | - |
- |
Lysinibacillus sphaericus |
| 5 | 8 | - |
Lactiplantibacillus plantarum |
| 5.5 | - |
- |
Enterobacter cloacae |
| 5.5 | - |
- |
Klebsiella pneumoniae |
| 5.5 | - |
- |
Enterobacter sp. |
| 6 | - |
- |
Rhodococcus sp. |
| 7 | - |
- |
Escherichia coli |
| 7 | - |
- |
Selenomonas ruminantium |
| 8.9 | - |
- |
Serratia ficaria |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Bacillus subtilis |
| 3 | 8 | - |
Lysinibacillus sphaericus |
| 4.5 | 5 | - |
Bacillus cereus |
| 5.5 | 8 | - |
Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | isoelectric focusing | - |
4.4 |
| Selenomonas ruminantium | isoelectric focusing | - |
6.58 |
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