Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) and B834(DE3) cells | Lactiplantibacillus plantarum |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M sodium acetate buffer (pH 5.0) containing 15-20% (w/v) polyethylene glycol 8000 and 0.2 M ammonium sulfate | Lactiplantibacillus plantarum |
Protein Variants | Comment | Organism |
---|---|---|
D421A | the mutant has 44fold lower activity than the wild type enzyme | Lactiplantibacillus plantarum |
E357A | the mutant has 230fold lower activity than the wild type enzyme | Lactiplantibacillus plantarum |
K343A | the mutant has very little activity compared to the wild type enzyme | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
methyl gallate | wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.95 | - |
methyl gallate | mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
1 | - |
methyl gallate | mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Lactiplantibacillus plantarum | - |
gallate + D-glucose | - |
? | |
tannic acid + H2O | Lactiplantibacillus plantarum SN35N | - |
gallate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | B3Y018 | - |
- |
Lactiplantibacillus plantarum SN35N | B3Y018 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Lactiplantibacillus plantarum SN35N | gallate + methanol | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus plantarum | gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus plantarum SN35N | gallate + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tannin acylhydrolase | - |
Lactiplantibacillus plantarum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
methyl gallate | mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
2.5 | - |
methyl gallate | mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
74 | - |
methyl gallate | wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
methyl gallate | mutant enzyme K343A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.52 | - |
methyl gallate | mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
2.7 | - |
methyl gallate | mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
120 | - |
methyl gallate | wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum |