Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2, 5 mM, no effect on the activity, both the free enzyme and the immoblized enzyme | Aspergillus oryzae | |
Co2+ | CoCl2 at 5 mM, free enzyme: 49.3% inhibition, immobilized enzyme: 24% inhibition | Aspergillus oryzae | |
Cu2+ | CuSO4 at 5 mM, free enzyme: 59% inhibition, immobilized enzyme: 44% inhibition | Aspergillus oryzae | |
FeSO4 | at 5 mM, free enzyme: 61.3% inhibition, immobilized enzyme: 28% inhibition | Aspergillus oryzae | |
HgCl2 | 5 mM, free enzyme: 13.7% inhibition, immobilized enzyme: 30% inhibition | Aspergillus oryzae | |
Mg2+ | MgCl2 at 5 mM, free enzyme: 28.4% inhibition, immobilized enzyme: 14% inhibition | Aspergillus oryzae | |
Zn2+ | ZnCl2 at 5 mM, free enzyme: 55.4% inhibition, immobilized enzyme: 29.3% inhibition | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Aspergillus oryzae | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.65 | - |
immobilized enzyme | Aspergillus oryzae |
72.2 | - |
free enzyme | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | - |
Aspergillus oryzae | gallic acid + ? | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae | 10 gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
free enzyme, activation energy 6.75 kcal per mol | Aspergillus oryzae |
55 | - |
immobilized enzyme, activation energy 5.77 kcal per mol | Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | immobilized enzyme, 25°C: 40% of the maximal activity, 75°C: 18% of the maximal activity. Free enzyme, 25°C: 57% of the maximal activity, 75°C: 10% of the maximal activity | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life of free enzyme: 90 min, half-life of immobilized enzyme: 120 min | Aspergillus oryzae |
60 | - |
half-life of free enzyme: 18 min, half-life of immobilized enzyme: 40 min | Aspergillus oryzae |
70 | - |
half-life of free enzyme: 8 min at, half-life of immobilized enzyme: 25 min | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
immobilized enzyme | Aspergillus oryzae |
5.5 | - |
free enzyme | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | immobilized enzyme, pH 3: 40% of the maximal activity, pH 8: 20% of the maximal activity. Free enzyme, pH 3: 5% of the maximal activity, pH 8: 20% of the maximal activity | Aspergillus oryzae |