Protein Variants | Comment | Organism |
---|---|---|
additional information | preparation of beta-ketoadipate:succinyl-coenzyme A transferase (PcaJ)- and beta-ketoadipate enol lactone hydrolase (PcaD)-inactivated mutants. A double mutant strain shows no beta-ketoadipate:succinyl-coenzyme A transferase and beta-ketoadipate enol lactone hydrolase activties and is unable to assimilate vanillate (VA) and protocatechuate (PCA), and is designated as Pseudomonas putida strain KT2440-dJ, phenotype, overview | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88N36 | - |
- |
Pseudomonas putida ATCC 47054 | Q88N36 | - |
- |
Pseudomonas putida DSM 6125 | Q88N36 | - |
- |
Pseudomonas putida NCIMB 11950 | Q88N36 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoadipate enol-lactone hydrolase | - |
Pseudomonas putida |
PcaD | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
malfunction | PcaJ (beta-ketoadipate:succinyl-coenzyme A transferase) inactivation results in production of 3-oxoadipate from protocatechuic acid. The mutant is a biocatalyst to produce both 3-oxoadipate and muconolactone from a lignin-related aromatic compound, a portion of the produced 3-oxoadipate is converted to levulinic acid through a purification step consisting of extraction from the culture and recrystallization. Muconolactone is purified from the culture of the PcaD-inactivated mutant of KT2440, although the isomeric beta-ketoadipate enol-lactone is supposed to be produced because it is the substrate of PcaD | Pseudomonas putida |
metabolism | the enzyme is part of the metabolic pathway for 4-hydroxphenyl and guaiacyl lignin-related aromatic compounds in Pseudomonas putida strain KT2440. 3-Carboxymuconolactone (CML), muconolactone (ML), and betaketoadipate enol-latone (KEL), i.e. 3-oxoadipate, are intermediates observed in the metabolism of lignin-related aromatics | Pseudomonas putida |