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Literature summary for 3.1.1.24 extracted from

  • Patel, R.N.; Mazumdar, S.; Ornston, L.N.
    beta-Ketoadipate enol-lactone hydrolases I and II from Acinetobacter calcoaceticus (1975), J. Biol. Chem., 250, 6567-6577.
    View publication on PubMed
Search for more literature for 3.1.1.24

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 2 * 12000, SDS-Page Acinetobacter calcoaceticus
25000
-
 ELH I and ELH II, gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O Acinetobacter calcoaceticus the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ELH I and II, 290fold, and 550fold Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
500
-
 ELH II Acinetobacter calcoaceticus
580
-
 ELH I Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Acinetobacter calcoaceticus 3-oxoadipate
-
 ?
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase Acinetobacter calcoaceticus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 12000, SDS-Page Acinetobacter calcoaceticus
More primarily as dimers, two further bands appear, the protein may associate to form higher oligomers Acinetobacter calcoaceticus

Synonyms

Synonyms Comment Organism
beta-ketoadipate enol-lactone hydrolase form I and II Acinetobacter calcoaceticus
ELH I protocatechuate-induced Acinetobacter calcoaceticus
ELH II cis, cis-muconate-induced Acinetobacter calcoaceticus