Any feedback?
Literature summary for 3.1.1.26 extracted from
- Purification and properties of a lipolytic acyl-hydrolase from potato leaves (1979), Biochim. Biophys. Acta, 573, 155-165.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 113% activity at 0.1 mM, 140% activity at 10 mM | Solanum tuberosum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | no inhibition at 0.1 mM, 13% inhibition at 1 mM, 47% inhibition at 10 mM | Solanum tuberosum | |
| MgCl2 | 53% inhibition at 10 mM | Solanum tuberosum | |
| NaCl | 84% inhibition at 50 mM | Solanum tuberosum | |
| SDS | 0.1% complete inhibition | Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
monogalactosyldiacylglycerol | - |
Solanum tuberosum | |
| 0.54 | - |
phosphatidylcholine | - |
Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
SDS-PAGE, gel filtration | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digalactosyldiacylglycerol + H2O | Solanum tuberosum | - |
? | - |
? | |
| monogalactosyldiacylglycerol + H2O | Solanum tuberosum | - |
? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 85% inhibition at 10% | Solanum tuberosum |
| Ethanol | 98% inhibition at 10% | Solanum tuberosum |
| ether | 96% inhibition at 10% | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
L. cv. Benimaru | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 520fold | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Solanum tuberosum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-di-O-acyl-1-O-(beta-D-galactosyl)-D-glycerol + H2O | - |
Solanum tuberosum | ? | - |
? | |
| digalactosyldiacylglycerol + H2O | 44% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | digalactosylglycerol + fatty acid | - |
? | |
| digalactosyldiacylglycerol + H2O | - |
Solanum tuberosum | ? | - |
? | |
| digalactosylmonoacylglycerol + H2O | 146% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | digalactosylglycerol + fatty acid | - |
? | |
| lysophosphatidylcholine + H2O | 26% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| lysophosphatidylethanolamine + H2O | 6% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| lysophosphatidylglycerol + H2O | 89% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| monogalactosyldiacylglycerol + H2O | - |
Solanum tuberosum | galactosylglycerol + fatty acid | - |
? | |
| monogalactosyldiacylglycerol + H2O | - |
Solanum tuberosum | ? | - |
? | |
| monogalactosylmonoacylglycerol + H2O | 394% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | monogalactosylglycerol + fatty acid | - |
? | |
| phosphatidic acid + H2O | 8% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| phosphatidylcholine + H2O | 14% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| phosphatidylethanolamine + H2O | 1% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| phosphatidylglycerol + H2O | 77% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| sulfoquinovosyldiaclglycerol + H2O | 43% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
| triacylglycerol + H2O | below 0.1% of the activity with monogalactosyldiacylglycerol | Solanum tuberosum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipid acyl-hydrolase | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
phosphatidylcholine as substrate | Solanum tuberosum |
| 35 | - |
monogalactosyldiacylglycerol as substrate | Solanum tuberosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
completely inactivated after 10 min at pH 8.0, 70% of its activity retained at pH 5.5 | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
monogalactosyldiacylglycerol as substrate, at 35°C, shift to pH 6.0 in the presence of Triton X-100 | Solanum tuberosum |
| 6 | - |
monogalactosyldiacylglycerol as substrate, at 35°C, shift from pH 5.5 in the presence of Triton X-100 | Solanum tuberosum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
