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Literature summary for 3.1.1.3 extracted from

  • Bhardwaj, K.; Raju, A.; Rajasekharan, R.
    Identification, purification, and characterization of a thermally stable lipase from rice bran. A new member of the (phospho) lipase family (2001), Plant Physiol., 127, 1728-1738.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
potassium acetate 20-200 mM, 4-5fold increase in activity Oryza sativa
Sodium acetate 20 mM, 2fold increase in activity, no effect at higher concentration at pH 7.5 Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ about 70% inhibition at 2 mM Oryza sativa
CHAPS inhibitory at 0.5 mM Oryza sativa
Cu2+ about 70% inhibition at 2 mM Oryza sativa
Digitonin inhibitory at 0.5 mM Oryza sativa
diisopropyl fluorophosphate inhibits both lipase and phospholipase activities of the enzyme Oryza sativa
Mg2+ about 70% inhibition at 2 mM Oryza sativa
additional information no inhibition by Ca2+, Triton X-100 and NP-40 decreased the activity marginally at lower concentrations and then restorde the activity at 4 mM Oryza sativa
SDS 50% inhibition at 2mM Oryza sativa
Zn2+ about 70% inhibition at 2 mM Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.02
-
 phosphocholine pH 7.5, 37°C Oryza sativa
6.71
-
 triolein pH 7.5, 37°C Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9400
-
 gel filtration and MALDI Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Oryza sativa

Purification (Commentary)

Purification (Comment) Organism
7.6fold to homogeneity Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
seed coat
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00012
-
 purified enzyme, phospholipase activity Oryza sativa
0.38
-
 purified enzyme, lipase activity Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacylglycerol + H2O
-
 Oryza sativa fatty acid + acylglycerol
-
 ?
additional information enzyme also provides phospholipase A2 activity, it preferentially hydrolyzes the sn-2-position of the substrate Oryza sativa ?
-
 ?
phosphatidylethanolamine + H2O
-
 Oryza sativa ?
-
 ?
phosphocholine + H2O
-
 Oryza sativa ?
-
 ?
triolein + H2O enzyme shows no positional specificity towards the triacylglycerol Oryza sativa diolein + oleate
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 90 completely stable up to 40°C, 65% remaining activity at 60°C after 1 h, 76°C is the transition temperature, the enzyme is still active and retains most of its secondary structure at 90°C, 34% remaining activity at 90°C after 15 min pretreatment at 90°C, 16% remaining activity at 37°C after 15 min pretreatment at 90°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
 Oryza sativa