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Literature summary for 3.1.1.3 extracted from
- Val-407 and Ile-408 in the beta5'-loop of pancreatic lipase mediate lipase-colipase interactions in the presence of bile salt micelles (2006), J. Biol. Chem., 281, 7793-7800.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Pichia pastoris | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I408D | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
| I408K | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
| L412D | site-directed mutagenesis, slightly reduced activity compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
| L412K | site-directed mutagenesis, slightly reduced activity compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
| V407D | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
| V407K | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of beta5'-loop-mediated interaction of the enzyme with the colipase, overview | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Pichia pastoris | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3900 | - |
purified recombinant wild-type enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Val407 and Ile408 in the beta5'-loop are required for interaction of the enzyme with the colipase in presence of bile salt micelles, overview | Homo sapiens | ? | - |
? |  |
| tributyrin + H2O | - |
Homo sapiens | dibutyrin + butyrate | - |
? | |
| tricaprylin + H2O | - |
Homo sapiens | dicaprylin + caprylate | - |
? | |
| triolein + H2O | - |
Homo sapiens | diolein + oleate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure modelling of the beta5'-loop of wild-type and mutant enzymes from crystal structure, PDB code 1LPA | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pancreatic triglyceride lipase | - |
Homo sapiens |
| PTL | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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