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Literature summary for 3.1.1.3 extracted from

  • Huang, Y.; Locy, R.; Weete, J.D.
    Purification and characterization of an extracellular lipase from Geotrichum marinum (2004), Lipids, 39, 251-257.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate i.e. CHAPS, activates 3.6fold at 0.1 mM Geotrichum marinum
taurocholic acid activates 50% at 0.1 mM Geotrichum marinum

Inhibitors

Inhibitors Comment Organism Structure
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate i.e. CHAPS, 33% inhibition at 1 mM Geotrichum marinum
cetyltriethylammonium bromide complete inhibition at 1 mM Geotrichum marinum
Fe2+ inhibits at 6 mM Geotrichum marinum
methylbenzethonium bromide 98% inhibition at 1 mM Geotrichum marinum
Mn2+ inhibits at 6 mM Geotrichum marinum
SDS complete inhibition at 1 mM Geotrichum marinum
sodium dioctyl sulfosuccinate 47% inhibition at 1 mM Geotrichum marinum
Triton X-100 complete inhibition at 1 mM Geotrichum marinum
Tween 20 complete inhibition at 1 mM Geotrichum marinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.5
-
triolein pH 7.5, 40°C Geotrichum marinum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Geotrichum marinum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Geotrichum marinum
K+ stimulates at 6 mM, inhibits at 500 mM Geotrichum marinum
Mg2+ stimulates Geotrichum marinum
Na+ stimulates at 6 mM, inhibits at 500 mM Geotrichum marinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
gel filtration Geotrichum marinum

Organism

Organism UniProt Comment Textmining
Geotrichum marinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 76fold by affinity chromatography on octyl-resin, and gel filtration Geotrichum marinum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
773
-
purified enzyme Geotrichum marinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, the extracellular enzyme prefers triacylglyceride substrates with cis double bonds in the fatty acid side chains, activity increases with number of double bonds, no activity with tristearin Geotrichum marinum ?
-
?
trielaidin + H2O lower activity Geotrichum marinum dielaidin + elaidic acid
-
?
trilinolein + H2O good substrate Geotrichum marinum dilinolein + linoleate
-
?
trilinolenin + H2O preferred substrate Geotrichum marinum dilinolenin + linolenate
-
?
triolein + H2O good substrate Geotrichum marinum diolein + oleate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 62000, SDS-PAGE Geotrichum marinum

Synonyms

Synonyms Comment Organism
extracellular lipase
-
Geotrichum marinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Geotrichum marinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50
-
Geotrichum marinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the purified extracellular enzyme is fully stable for 1 h, but loses 48% and 51% of its activity after 4 h at 20°C and 40°C, respectively Geotrichum marinum
60
-
inactivation above Geotrichum marinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geotrichum marinum

pH Range

pH Minimum pH Maximum Comment Organism
5 10 20% of maximal activity at pH 5.0, 80% at pH 10.0, inactive at pH 4.0 Geotrichum marinum

pI Value

Organism Comment pI Value Maximum pI Value
Geotrichum marinum isoelectric focusing
-
4.5