Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | i.e. CHAPS, activates 3.6fold at 0.1 mM | Geotrichum marinum | |
taurocholic acid | activates 50% at 0.1 mM | Geotrichum marinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | i.e. CHAPS, 33% inhibition at 1 mM | Geotrichum marinum | |
cetyltriethylammonium bromide | complete inhibition at 1 mM | Geotrichum marinum | |
Fe2+ | inhibits at 6 mM | Geotrichum marinum | |
methylbenzethonium bromide | 98% inhibition at 1 mM | Geotrichum marinum | |
Mn2+ | inhibits at 6 mM | Geotrichum marinum | |
SDS | complete inhibition at 1 mM | Geotrichum marinum | |
sodium dioctyl sulfosuccinate | 47% inhibition at 1 mM | Geotrichum marinum | |
Triton X-100 | complete inhibition at 1 mM | Geotrichum marinum | |
Tween 20 | complete inhibition at 1 mM | Geotrichum marinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.5 | - |
triolein | pH 7.5, 40°C | Geotrichum marinum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geotrichum marinum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Geotrichum marinum | |
K+ | stimulates at 6 mM, inhibits at 500 mM | Geotrichum marinum | |
Mg2+ | stimulates | Geotrichum marinum | |
Na+ | stimulates at 6 mM, inhibits at 500 mM | Geotrichum marinum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Geotrichum marinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geotrichum marinum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 76fold by affinity chromatography on octyl-resin, and gel filtration | Geotrichum marinum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
773 | - |
purified enzyme | Geotrichum marinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, the extracellular enzyme prefers triacylglyceride substrates with cis double bonds in the fatty acid side chains, activity increases with number of double bonds, no activity with tristearin | Geotrichum marinum | ? | - |
? | |
trielaidin + H2O | lower activity | Geotrichum marinum | dielaidin + elaidic acid | - |
? | |
trilinolein + H2O | good substrate | Geotrichum marinum | dilinolein + linoleate | - |
? | |
trilinolenin + H2O | preferred substrate | Geotrichum marinum | dilinolenin + linolenate | - |
? | |
triolein + H2O | good substrate | Geotrichum marinum | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, SDS-PAGE | Geotrichum marinum |
Synonyms | Comment | Organism |
---|---|---|
extracellular lipase | - |
Geotrichum marinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Geotrichum marinum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Geotrichum marinum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the purified extracellular enzyme is fully stable for 1 h, but loses 48% and 51% of its activity after 4 h at 20°C and 40°C, respectively | Geotrichum marinum |
60 | - |
inactivation above | Geotrichum marinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geotrichum marinum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 20% of maximal activity at pH 5.0, 80% at pH 10.0, inactive at pH 4.0 | Geotrichum marinum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geotrichum marinum | isoelectric focusing | - |
4.5 |