Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CTAB | PFL immobilized on glyoxyl and BrCN agarose is activates 8fold at 0.01% CTAB | Pseudomonas fluorescens | |
additional information | detergents mediate hyperactivation, effect of the detergents is to shift the conformational equilibrium of lipases toward the open form | Pseudomonas fluorescens | |
additional information | detergents mediate hyperactivation, effect of the detergents is to shift the conformational equilibrium of lipases toward the open form | Moesziomyces antarcticus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the enzyme on glyoxyl-agarose at pH 10.0 and 25°C and on BrCN agarose, overview | Pseudomonas fluorescens |
additional information | immobilization of the enzyme on glyoxyl-agarose at pH 10.0 and 25°C and on BrCN agarose, overview | Moesziomyces antarcticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride | the presence of detergents, e.g. SDS, CTAB, Triton X-100, or Triton X-45, permitts the inhibition of lipase by irreversible covalent inhibitors, e.g. 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF, while the enzyme, in the absence of detergent, is not inhibited by these irreversible inhibitors | Moesziomyces antarcticus | |
4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride | the presence of detergents, e.g. SDS, CTAB, Triton X-100, or Triton X-45, permits the inhibition of lipase by irreversible covalent inhibitors, e.g. 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF, while the enzyme, in the absence of detergent, is not inhibited by these irreversible inhibitors | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moesziomyces antarcticus | - |
isozyme b | - |
Pseudomonas fluorescens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Pseudomonas fluorescens | - |
commercial preparation | - |
Moesziomyces antarcticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of the enzyme immobilized on glyoxyl-agarose and on BrCN agarose, overview | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+-)-2-hydroxy-4-phenylbutyric acid ethyl ester + H2O | enantioselectivity of the immobilized enzyme in this reaction is increased 40fold by presence of 0.1% CTAB | Pseudomonas fluorescens | ? | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Moesziomyces antarcticus | 4-nitrophenol + palmitate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
25 | - |
assay at | Moesziomyces antarcticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas fluorescens |
7 | - |
assay at | Moesziomyces antarcticus |