Protein Variants | Comment | Organism |
---|---|---|
D20N/S53P/S155M/L162G/T180I/T234S | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L159W/L162T | site-directed mutagenesis, the mutant shows higher enantioselectivity as the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162A | site-directed mutagenesis, the mutant shows higher enantioselectivity as the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162D | site-directed mutagenesis, the mutant shows higher enantioselectivity as the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162F | site-directed mutagenesis, a highly enantioselective mutant, use in combinatorial active site saturation test for kinetic resolution of an axially chiral allene, 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate, the high enantioselectivity of the mutant is rationalized by PIPI stacking | Pseudomonas aeruginosa |
L162G | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162G/L159V | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162G/L159Y | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162I | site-directed mutagenesis, the mutant shows higher enantioselectivity as the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162T | site-directed mutagenesis, the mutant shows higher enantioselectivity as the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L162V | site-directed mutagenesis, the mutant shows higher enantioselectivity as the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L17F | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
L231e/V232C | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
M16A | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
V232I | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
V232I/M16L/A34T/P86L/D113G/S237T/T150A/S147N/V94A/T87S/L208H | site-directed mutagenesis, the mutant shows reduced enantioselectivity compared to the wild-type enzyme with substrates 4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate and 4-nitrophenyl 2-methyldecanoate | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
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Pseudomonas aeruginosa | - |
- |
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Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
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additional information | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl 2-methyldecanoate + H2O | kinetic resolution of enantioselectivity, overview | Pseudomonas aeruginosa | ? | - |
? | |
4-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate + H2O | reaction via R-tetrahedral intermediate and S-tetrahedral intermediate, overview, the wild-type lipase and the mutant L162F show preference for the (+)-enantiomer, the mutant shows high enantioselectivity, kinetic resolution of enantioselectivity, overview | Pseudomonas aeruginosa | ? | - |
? |