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Literature summary for 3.1.1.3 extracted from
- Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05 (2007), J. Mol. Catal. B, 49, 28-35.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ggl, cloning of both genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, sequence comparisons | Geotrichum candidum |
| gene ggl, cloning of both genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression in Pichia pastoris strain GS115 under the control of the methanol inducible alcohol oxidase promoter AOX1, seceretion of the recombinant lipase | Geotrichum candidum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
x * 64000, recombinant enzyme, SDS-PAGE | Geotrichum candidum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geotrichum candidum | - |
- |
- |
| Geotrichum candidum | Q0MVP3 | i.e. Oospora lactis or Dipodascus geotrichum | - |
| Geotrichum candidum Y05 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | GGL contains potential glycosylation sites | Geotrichum candidum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GGL 3.2fold from Pichia pastoris strain GS115 culture supernatant | Geotrichum candidum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad is formed by Ser236, Glu373, and His482 | Geotrichum candidum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geotrichum candidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| castor oil + H2O | - |
Geotrichum candidum | ? | - |
? |  |
| castor oil + H2O | - |
Geotrichum candidum Y05 | ? | - |
? | |
| corn oil + H2O | - |
Geotrichum candidum | ? | - |
? | |
| corn oil + H2O | - |
Geotrichum candidum Y05 | ? | - |
? | |
| cotton seed oil + H2O | - |
Geotrichum candidum | ? | - |
? | |
| lard + H2O | - |
Geotrichum candidum | ? | - |
? | |
| additional information | substrate specificity with natural oils and triacylglycerol substrates, overview, the enzyme catalyzes the hydrolysis of triacylglycerols into fatty acids and glycerol at the water-lipid interface and its reverse reaction in non-aqueous solvents | Geotrichum candidum | ? | - |
? | |
| additional information | the enzyme catalyzes the hydrolysis of triacylglycerols into fatty acids and glycerol at the water-lipid interface and its reverse reaction in non-aqueous solvents | Geotrichum candidum | ? | - |
? | |
| additional information | substrate specificity with natural oils and triacylglycerol substrates, overview, the enzyme catalyzes the hydrolysis of triacylglycerols into fatty acids and glycerol at the water-lipid interface and its reverse reaction in non-aqueous solvents | Geotrichum candidum Y05 | ? | - |
? | |
| olive oil + H2O | - |
Geotrichum candidum | ? | - |
? | |
| olive oil + H2O | - |
Geotrichum candidum Y05 | ? | - |
? | |
| rape seed oil + H2O | - |
Geotrichum candidum | ? | - |
? | |
| soybean oil + H2O | - |
Geotrichum candidum | ? | - |
? | |
| soybean oil + H2O | - |
Geotrichum candidum Y05 | ? | - |
? | |
| tea oil + H2O | - |
Geotrichum candidum | ? | - |
? | |
| tung oil + H2O | - |
Geotrichum candidum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, recombinant enzyme, SDS-PAGE | Geotrichum candidum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GGL | - |
Geotrichum candidum |
| lipase | - |
Geotrichum candidum |
| triacylglycerol acylhydrolase | - |
Geotrichum candidum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | - |
Geotrichum candidum |
| 50 | - |
- |
Geotrichum candidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable at least for 12 h at pH 8.0 below 60°C, above 60°C rapid inactivation occurs with only 36% residual activity | Geotrichum candidum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Geotrichum candidum |
| 8 | - |
- |
Geotrichum candidum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | purified recombinant enzyme, more than 60% residual activity after incubation at pH 6.0-10.0 for 24 h at room temperature | Geotrichum candidum |
| 11 | - |
purified recombinant enzyme, 19% residual activity after incubation for 24 h at room temperature | Geotrichum candidum |
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