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Literature summary for 3.1.1.3 extracted from

  • Singh, S.; Banerjee, U.C.
    Purification and characterization of trans-3-(4-methoxyphenyl) glycidic acid methyl ester hydrolyzing lipase from Pseudomonas aeruginosa (2007), Process Biochem., 42, 1063-1068.
No PubMed abstract available
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not affected by EDTA and Tween 80 Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
DTT
-
 Pseudomonas aeruginosa
additional information the enzyme is not affected by EDTA and Tween 80 Pseudomonas aeruginosa
SDS
-
 Pseudomonas aeruginosa
Triton X-100
-
 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
50
-
 4-nitrophenyl palmitate pH 8.0, 30°C Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59400
-
 x * 59400, SDS-PAGE Pseudomonas aeruginosa
300000
-
 gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 isolated from the soil
-
Pseudomonas aeruginosa MTCC 5113
-
 isolated from the soil
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 21fold from the culture supernatant by acetone precipitation, anion exchange chromatography and gel filtration Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19280
-
 purified enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa MTCC 5113 4-nitrophenol + palmitate
-
 ?
trans-3-(4-methoxyphenyl) glycidic acid methyl ester + H2O i.e. (+-)-methyl trans-3(4-methoxyphenyl) glycidate, the substrate is a key intermediate in the synthesis of cardiovascular drug, diltiazem Pseudomonas aeruginosa ?
-
 ?
trans-3-(4-methoxyphenyl) glycidic acid methyl ester + H2O i.e. (+-)-methyl trans-3(4-methoxyphenyl) glycidate, the substrate is a key intermediate in the synthesis of cardiovascular drug, diltiazem Pseudomonas aeruginosa MTCC 5113 ?
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 59400, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
enantiospecific lipase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 30°C, 48 h, purified enzyme, loss of 33% activity Pseudomonas aeruginosa
7 9 30°C, 48 h, purified enzyme, completely stable Pseudomonas aeruginosa
10
-
 30°C, 3 h, purified enzyme, loss of 50% activity Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa isoelectric focusing
-
 5.5