Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Geobacillus thermoleovorans | |
DTT | - |
Geobacillus thermoleovorans |
Cloned (Comment) | Organism |
---|---|
gene lipA, DNA and amino acid sequence determination and anaylsis, phylogenetic analysis, expression of His6-tagged enzyme in Escherichia coli strain DH5alpha under control of the temperature-inducible lambda-promoter | Geobacillus thermoleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Isopropanol | slight inhibition at 1% | Geobacillus thermoleovorans | |
additional information | poor inhibition by Mg2+ at 1 mM, and by 2-mercaptoethanol, DTT, SDS, and EDTA at 1% | Geobacillus thermoleovorans | |
Zn2+ | 86% inhibition at 1 mM | Geobacillus thermoleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
4-nitrophenyl palmitate | pH 7.5,60°C | Geobacillus thermoleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation | Geobacillus thermoleovorans | |
DMSO | slight activation | Geobacillus thermoleovorans | |
additional information | no effect by Cs+ | Geobacillus thermoleovorans | |
Na+ | slight activation | Geobacillus thermoleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 46000, pre-protein, SDS-PAGE, x * 43000, mature LipA, SDS-PAGE | Geobacillus thermoleovorans |
46000 | - |
x * 46000, pre-protein, SDS-PAGE, x * 43000, mature LipA, SDS-PAGE | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | - |
isolated from desert soil in Egypt, gene lipA | - |
Geobacillus thermoleovorans YN | - |
isolated from desert soil in Egypt, gene lipA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme contains a signal sequence of 28 amino acids | Geobacillus thermoleovorans |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 130fold from Escherichia coli by immobilized metal-affinity chromatography and gel filtration | Geobacillus thermoleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3586 | - |
purified recombinant lipase | Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Geobacillus thermoleovorans YN | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | best 4-nitrophenyl ester substrate | Geobacillus thermoleovorans | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Geobacillus thermoleovorans YN | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Geobacillus thermoleovorans YN | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Geobacillus thermoleovorans YN | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + stearate | - |
? | |
additional information | substrate specificity, methyl eicosanoate and methyl decosanoate are poor substrates, overview | Geobacillus thermoleovorans | ? | - |
? | |
additional information | substrate specificity, methyl eicosanoate and methyl decosanoate are poor substrates, overview | Geobacillus thermoleovorans YN | ? | - |
? | |
triacetin + H2O | - |
Geobacillus thermoleovorans | diacetin + acetate | - |
? | |
tributyrin + H2O | - |
Geobacillus thermoleovorans | dibutyrin + butyrate | - |
? | |
tricaprylin + H2O | best triacylglyceride substrate | Geobacillus thermoleovorans | dicaprylin + caprylate | - |
? | |
trimyristin + H2O | - |
Geobacillus thermoleovorans | dimyristin + myristate | - |
? | |
tripalmitin + H2O | high activity | Geobacillus thermoleovorans | dipalmitin + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, pre-protein, SDS-PAGE, x * 43000, mature LipA, SDS-PAGE | Geobacillus thermoleovorans |
Synonyms | Comment | Organism |
---|---|---|
LipA | - |
Geobacillus thermoleovorans |
LipAB | - |
Geobacillus thermoleovorans |
lipase | - |
Geobacillus thermoleovorans |
lipase A | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | recombinant enzyme | Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, purified recombinant enzyme, stable up to | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
recombinant enzyme | Geobacillus thermoleovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
purified recombinant enzyme, stable, at 60°C | Geobacillus thermoleovorans |
10.5 | - |
purified recombinant enzyme, stable, at 60°C | Geobacillus thermoleovorans |