Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Chen, S.; Qian, L.; Shi, B.
    Purification and properties of enantioselective lipase from a newly isolated Bacillus cereus C71 (2007), Process Biochem., 42, 988-994.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100
-
Bacillus cereus
Tween-20
-
Bacillus cereus
Tween-40
-
Bacillus cereus
Tween-80
-
Bacillus cereus

Application

Application Comment Organism
synthesis the enantioselective lipase shows potential to be used as a catalyst to prepare optically pure pharmaceuticals Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Bacillus cereus
Cu2+
-
Bacillus cereus
K+
-
Bacillus cereus
Mg2+
-
Bacillus cereus
Mn2+
-
Bacillus cereus
Ni2+
-
Bacillus cereus
SDS
-
Bacillus cereus
Zn2+
-
Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme activity is not affected by Ca2+ or Na+ Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus C71
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain C71 1092fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and two steps of ion exchange chromatography Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
218.5
-
purified enzyme, substrate (R,S)-flurbiprofen ethyl ester Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-flurbiprofen ethyl ester + H2O enantioselective reaction Bacillus cereus (S)-flurbiprofen ethyl ester + (R)-flurbiprofen + ethanol
-
?
(R,S)-flurbiprofen ethyl ester + H2O enantioselective reaction Bacillus cereus C71 (S)-flurbiprofen ethyl ester + (R)-flurbiprofen + ethanol
-
?
ethyl 2-arylpropanoate + H2O
-
Bacillus cereus ethanol + (R)-2-arylpropanoate
-
?
ethyl 2-arylpropanoate + H2O
-
Bacillus cereus C71 ethanol + (R)-2-arylpropanoate
-
?
additional information substrate specificity, the enantioselective lipase hydrolyzes 4-nitrophenyl esters with acyl chain lengths between C4 and C12, and shows high enantioselectivity toward the R-isomer of ethyl 2-arylpropanoate, overview Bacillus cereus ?
-
?
additional information substrate specificity, the enantioselective lipase hydrolyzes 4-nitrophenyl esters with acyl chain lengths between C4 and C12, and shows high enantioselectivity toward the R-isomer of ethyl 2-arylpropanoate, overview Bacillus cereus C71 ?
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Bacillus cereus

Synonyms

Synonyms Comment Organism
enantioselective lipase
-
Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
33
-
-
Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 35 3 h, purified enzyme, 92% remaining activity at 35°C Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 10 purified enzyme, stable Bacillus cereus