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Literature summary for 3.1.1.3 extracted from
- Involvement of Gly 311 residue on substrate discrimination, pH and temperature dependency of recombinant Staphylococcus xylosus lipase: a study with emulsified substrate (2007), Protein Expr. Purif., 55, 31-39.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Staphylococcus xylosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G311D | site-directed mutagenesis, the mutant shows reduced activity and a distinct pH profile compared to the wild-type enzyme | Staphylococcus xylosus |
| G311K | site-directed mutagenesis, the mutant shows reduced activity and a distinct pH profile compared to the wild-type enzyme | Staphylococcus xylosus |
| G311L | site-directed mutagenesis, the mutant shows reduced activity but increased pH and thermal stability compared to the wild-type enzyme, the mutant also shows phospholipase activity with phosphatidylcholine in contrast to the wild-type enzyme | Staphylococcus xylosus |
| G311W | site-directed mutagenesis, inactive mutant | Staphylococcus xylosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus |  |
| 5 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus | |
| 13.9 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
| 18.9 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus | |
| 20.4 | - |
tributyrin | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus | |
| 27.8 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
| 35.7 | - |
olive oil | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus | |
| 100 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, recombinant enzyme, SDS-PAGE | Staphylococcus xylosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus xylosus | Q2TPV1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Staphylococcus xylosus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | residue Gly311 is essential for catalytic activity | Staphylococcus xylosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
purified recombinant mutant G311K, substrate olive oil | Staphylococcus xylosus |
| 25 | - |
purified recombinant mutant G311D, substrate olive oil | Staphylococcus xylosus |
| 30 | - |
purified recombinant mutant G311K, substrate tributyrin | Staphylococcus xylosus |
| 100 | - |
purified recombinant mutant G311D, substrate tributyrin | Staphylococcus xylosus |
| 220 | - |
purified recombinant mutant G311L, substrate olive oil | Staphylococcus xylosus |
| 280 | - |
purified recombinant mutant G311L, substrate tributyrin | Staphylococcus xylosus |
| 850 | - |
purified recombinant wild-type enzyme, substrate olive oil | Staphylococcus xylosus |
| 1500 | - |
purified recombinant wild-type enzyme, substrate tributyrin | Staphylococcus xylosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tributyrin + H2O | - |
Staphylococcus xylosus | dibutyrin + butyrate | - |
? | |
| trioctanoin + H2O | - |
Staphylococcus xylosus | dioctanoin + octanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, recombinant enzyme, SDS-PAGE | Staphylococcus xylosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Staphylococcus xylosus |
| SXL | - |
Staphylococcus xylosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Staphylococcus xylosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | - |
Staphylococcus xylosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant mutant G311L shows increased half-life of 280 min, while the mutant G311D and G311K show 30% and 35% remaining activity, respectively, after 30 min at 50°C | Staphylococcus xylosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
| 30 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
| 37.9 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus | |
| 50 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus | |
| 75.8 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus | |
| 250 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus | |
| 500 | - |
olive oil | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus | |
| 1500 | - |
tributyrin | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
mutant G311D | Staphylococcus xylosus |
| 8 | - |
wild-type enzyme | Staphylococcus xylosus |
| 9.5 | - |
mutant G311K | Staphylococcus xylosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | - |
Staphylococcus xylosus |
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