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Literature summary for 3.1.1.3 extracted from
- Protein-coated microcrystals of Pseudomonas aeruginosa PseA lipase (2008), Appl. Biochem. Biotechnol., 151, 160-166.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | lipases constitute one of the most important groups of biocatalysts due to their ability to catalyze three different kinds of reactions viz. hydrolysis, esterification, and transesterification with unique chemo-, regio-, and enantiospecific selectivity. Lipase-based processes are employed for oil/fat processing, synthesis of industrially important oleochemicals, enantiopure pharmaceuticals, agrochemicals, flavor esters, structured lipids, and biodiesel production | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | preparation of highly active lipase protein-coated microcrystals, PAL PCMC, by immobilization of protein onto K2SO4 as excipient solid support carrier and n-propanol as precipitating solvent, the Vmax, Km, and temperature optimum for PAL PCMC are increased compared to the soluble enzyme, PAL PCMC exhibits an 11.5fold increase in rate of hydrolysis with 4-nitrophenyl palmitate and 200fold with 1-phenylethanol compared to free enzyme, also the immobilized enzyme is thermally more stable with 4.65, 2.56, and 1.24fold improvement in half-lives at 45°C, 55°C, and 60°C compared to free PseA lipase, overview | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 589 | - |
4-nitrophenyl palmitate | free enzyme | Pseudomonas aeruginosa |  |
| 679.8 | - |
4-nitrophenyl palmitate | immobilied enzyme | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| triacylglycerol + H2O | Pseudomonas aeruginosa | - |
diacylglycerol + a carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | the immobilized enzyme PAL PCMC works as better catalyst for hydrolysis of pNPP in n-heptane medium | Pseudomonas aeruginosa | palmitate + 4-nitrophenol | - |
? | |
| butyl butyrate + H2O | - |
Pseudomonas aeruginosa | butane + butyrate | - |
? | |
| ethyl butyrate + H2O | - |
Pseudomonas aeruginosa | ethane + butyrate | - |
? | |
| triacylglycerol + H2O | - |
Pseudomonas aeruginosa | diacylglycerol + a carboxylate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Pseudomonas aeruginosa |
| PseA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
free enzyme | Pseudomonas aeruginosa |
| 45 | - |
immobilied enzyme | Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | - |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Pseudomonas aeruginosa |
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