Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Singh, P.K.; Gupta, M.N.
    Simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan (2008), Biochim. Biophys. Acta, 1784, 1825-1829.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain ER2566 as recombinant enzyme with intein tag Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
4-nitrophenyl palmitate native enzyme, at pH 7 and 37°C Burkholderia cepacia
0.12
-
4-nitrophenyl palmitate recombinant enzyme, at pH 7 and 37°C Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
recombinant enzyme, SDS-PAGE Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chitin column chromatography and chitosan affinity precipitation (0.3 (w/v) chitosan is required for obtaining maximum active enzyme) Burkholderia cepacia

Renatured (Commentary)

Renatured (Comment) Organism
the intein tag is cleaved with dithiothreitol and the refolded lipase is obtained in active form (activity recovery of 80%) Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2960
-
refolded and purified enzyme, at pH 7 and 37°C Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl palmitate + H2O
-
Burkholderia cepacia 4-nitrophenol + palmitate
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Burkholderia cepacia