Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain ER2566 as recombinant enzyme with intein tag | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-nitrophenyl palmitate | native enzyme, at pH 7 and 37°C | Burkholderia cepacia | |
0.12 | - |
4-nitrophenyl palmitate | recombinant enzyme, at pH 7 and 37°C | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
recombinant enzyme, SDS-PAGE | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chitin column chromatography and chitosan affinity precipitation (0.3 (w/v) chitosan is required for obtaining maximum active enzyme) | Burkholderia cepacia |
Renatured (Comment) | Organism |
---|---|
the intein tag is cleaved with dithiothreitol and the refolded lipase is obtained in active form (activity recovery of 80%) | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2960 | - |
refolded and purified enzyme, at pH 7 and 37°C | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + palmitate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Burkholderia cepacia |