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Literature summary for 3.1.1.3 extracted from
- Membrane-bound synthetic lipase specifically cultured under solid-state fermentation and submerged fermentation by Rhizopus chinensis: A comparative investigation (2008), Bioresour. Technol., 100, 1336-1342.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | SML shows 16% increased activity in the presence of 10 mM Al3+ | Rhizopus microsporus var. chinensis |  |
| K+ | SSL shows 22% increased activity in the presence of 10 mM K+ | Rhizopus microsporus var. chinensis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | SSL shows 67% residual activity in the presence of 10 mM Al3+ | Rhizopus microsporus var. chinensis | |
| Ca2+ | SSL and SML show 80% and 60% residual activity, respectively, in the presence of 10 mM Ca2+ | Rhizopus microsporus var. chinensis | |
| Cu2+ | SSL and SML show 5% and 30% residual activity, respectively, in the presence of 10 mM Cu2+ | Rhizopus microsporus var. chinensis | |
| Fe2+ | SSL and SML show 73% and 18% residual activity, respectively, in the presence of 10 mM Fe2+ | Rhizopus microsporus var. chinensis | |
| Fe3+ | SSL and SML show 28% and 9% residual activity, respectively, in the presence of 10 mM Fe3+ | Rhizopus microsporus var. chinensis | |
| Hg2+ | SSL and SML show 7% and 9% residual activity, respectively, in the presence of 10 mM Hg2+ | Rhizopus microsporus var. chinensis | |
| K+ | SML shows 85% residual activity in the presence of 10 mM Ki+ | Rhizopus microsporus var. chinensis | |
| Li+ | SSL and SML show 80% and 60% residual activity, respectively, in the presence of 10 mM Li+ | Rhizopus microsporus var. chinensis | |
| Mg2+ | SSL and SML show 54% and 71% residual activity, respectively, in the presence of 10 mM Mg2+ | Rhizopus microsporus var. chinensis | |
| Mn2+ | SSL and SML show 80% and 60% residual activity, respectively, in the presence of 10 mM Mn2+ | Rhizopus microsporus var. chinensis | |
| additional information | not affected by 2-mercaptoethanol and EDTA | Rhizopus microsporus var. chinensis | |
| phenylmethylsulfonyl fluoride | SML shows 32% residual activity in the presence of 10 mM phenylmethylsulfonyl fluoride | Rhizopus microsporus var. chinensis | |
| SDS | SDS shows strong inhibitory effect on SSL and SML, leaving residual activity of 43% and 28%, respectively | Rhizopus microsporus var. chinensis | |
| Zn2+ | SSL and SML show 76% and 55% residual activity, respectively, in the presence of 10 mM Zn2+ | Rhizopus microsporus var. chinensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SML, calculated from amino acid sequence | Rhizopus microsporus var. chinensis |
| 63000 | - |
SSL, calculated from amino acid sequence | Rhizopus microsporus var. chinensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus microsporus var. chinensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, butyl Sepharose column chromatography, and Superdex 75 gel filtration | Rhizopus microsporus var. chinensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
crude SSL, at 37°C | Rhizopus microsporus var. chinensis |
| 11.5 | - |
crude SML, at 37°C | Rhizopus microsporus var. chinensis |
| 48.8 | - |
SML after 4.2fold purification, at 37°C | Rhizopus microsporus var. chinensis |
| 305 | - |
SSL after 63.5fold purification, at 37°C | Rhizopus microsporus var. chinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Rhizopus microsporus var. chinensis | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Rhizopus microsporus var. chinensis | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caproate + H2O | - |
Rhizopus microsporus var. chinensis | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Rhizopus microsporus var. chinensis | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Rhizopus microsporus var. chinensis | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl palmitate + H2O | preferred substrate | Rhizopus microsporus var. chinensis | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Rhizopus microsporus var. chinensis | 4-nitrophenol + propionate | - |
? | |
| 4-nitrophenyl stearate + H2O | - |
Rhizopus microsporus var. chinensis | 4-nitrophenol + stearate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Rhizopus microsporus var. chinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SML | lipase obtained from submerged fermentation cell culture | Rhizopus microsporus var. chinensis |
| SSL | lipase obtained from solid-state fermentation cell culture | Rhizopus microsporus var. chinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Rhizopus microsporus var. chinensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | - |
Rhizopus microsporus var. chinensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | SSL shows 57% and 65.2% of its maximum activity at 30°C and 50°C, SML displays approximately 75.9% of its maximal activity at 30°C, SSL retains greater than 80% of the initial activity after 1 h incubation from 30 to 50°C, but the activity is reduced to 44.3% after exposure to 60°C for 1 h, SML is only stable below 40°C, the hydrolytic activity of purified SML decreases dramatically when the temperature increased above 50°C, and 49.7% and 84.1% of the activity is lost at 50 and 60°C after 1 h exposure, SML almost loses all of the original activity at 70°C after 1 h incubation | Rhizopus microsporus var. chinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhizopus microsporus var. chinensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Rhizopus microsporus var. chinensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | more than 80% of the initial activity is conserved at these pHs when incubated at 30°C for 1 h, over 70% of hydrolytic activity is retained at pH 7.0-8.0 for purified SML after 1 h of incubation, the enzyme stability decreases sharply at pH 9.0 and loses 61.7% activity at such pH value, SSL and SML are strongly inhibited by extreme acidic pH (under 5) | Rhizopus microsporus var. chinensis |
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