Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Pseudomonas fragi |
Protein Variants | Comment | Organism |
---|---|---|
D2Y/H30N | mutant shows elevated thermal stability with half-lives of 125 min at 37°C and 44 min at 42°C | Pseudomonas fragi |
R127G/R152H | mutant shows elevated thermal stability with half-lives of 98 min at 37°C and 38 min at 42°C | Pseudomonas fragi |
R127G/R152H/D2Y/H30N | mutant shows elevated thermal stability with half-lives of more than 4 h at 37°C and more than 100 min at 42°C | Pseudomonas fragi |
R127G/R152H/K205Q/S271T | mutant shows elevated thermal stability with half-lives of 154 min at 37°C and 47 min at 42°C | Pseudomonas fragi |
R127G/R152H/R70S/V121I/T131K/K205Q/S271T | mutant shows elevated thermal stability with half-lives of 132 min at 37°C and 52 min at 42°C | Pseudomonas fragi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fragi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyceryl trioctanoate + H2O | - |
Pseudomonas fragi | glycerol + octanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Pseudomonas fragi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas fragi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 42 | the wild type enzyme displays high activity at 10°C, but it is easily inactivated with half-lives of 4 h at 29°C, 83 min at 37°C, and 19 min at 42°C | Pseudomonas fragi |