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Literature summary for 3.1.1.3 extracted from
- Gene cloning, expression, and characterization of the Geobacillus thermoleovorans CCR11 thermoalkaliphilic lipase (2008), Mol. Biotechnol., 42, 75-83.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Geobacillus thermoleovorans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
mature lipase, SDS-PAGE | Geobacillus thermoleovorans |
| 42000 | - |
mature lipase, calculated from amino acid sequence | Geobacillus thermoleovorans |
| 57000 | - |
recombinant biotinylated enzyme, SDS-PAGE | Geobacillus thermoleovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermoleovorans | Q0JRM8 | - |
- |
| Geobacillus thermoleovorans CCR11 | Q0JRM8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl decanoate + H2O | preferred substrate of the native mature enzyme | Geobacillus thermoleovorans | 4-nitrophenol + decanoate | - |
? |  |
| 4-nitrophenyl decanoate + H2O | preferred substrate of the native mature enzyme | Geobacillus thermoleovorans CCR11 | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Geobacillus thermoleovorans CCR11 | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl octanoate + H2O | preferred substrate of the recombinant mature lipase | Geobacillus thermoleovorans | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | preferred substrate of the recombinant mature lipase | Geobacillus thermoleovorans CCR11 | 4-nitrophenol + octanoate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LipMatCCR11 lipase | mature lipase protein | Geobacillus thermoleovorans |
| triacylglycerol hydrolase | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant mature lipase | Geobacillus thermoleovorans |
| 60 | - |
native enzyme | Geobacillus thermoleovorans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | native enzyme | Geobacillus thermoleovorans |
| 30 | 60 | recombinant mature lipase | Geobacillus thermoleovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
recombinant mature lipase | Geobacillus thermoleovorans |
| 9 | 10 | native enzyme | Geobacillus thermoleovorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | native enzyme | Geobacillus thermoleovorans |
| 7 | 10 | recombinant mature lipase | Geobacillus thermoleovorans |
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