Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl 4-nitrophenyl phosphate | 10% residual activity at 10 mM | Penaeus vannamei | |
tetrahydrolipstatin | complete inhibition at 1 mM | Penaeus vannamei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.03 | - |
4-Methylumbelliferyl butyrate | apparent value, in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Penaeus vannamei | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the lipase is not dependent on Ca2+ for activation or stability | Penaeus vannamei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63360 | - |
1 * 95260 + 1 * 63360, calculated from SDS-PAGE | Penaeus vannamei |
95260 | - |
1 * 95260 + 1 * 63360, calculated from SDS-PAGE | Penaeus vannamei |
196000 | - |
gel filtration | Penaeus vannamei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus vannamei | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme lacks glycosylation | Penaeus vannamei |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography, gel filtration | Penaeus vannamei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive gland | - |
Penaeus vannamei | - |
pleopod | - |
Penaeus vannamei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.83 | - |
crude extract, using triolein as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
27.2 | - |
purified enzyme, using 2-naphthyl caprylate as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
71.5 | - |
purified enzyme, using 2-naphthyl acetate as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
85.1 | - |
purified enzyme, using 1-naphthyl acetate as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
213.6 | - |
purified enzyme, using tripalmitin as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
215 | - |
purified enzyme, using tributyrin as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
416.5 | - |
purified enzyme, using tristearin as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
922.2 | - |
after 58.25fold purification, using triolein as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Penaeus vannamei | 1-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Penaeus vannamei | 2-naphthol + acetate | - |
? | |
2-naphthyl caprylate + H2O | - |
Penaeus vannamei | 2-naphthol + caprylate | - |
? | |
4-methylumbelliferyl butyrate + H2O | - |
Penaeus vannamei | 4-methylumbelliferone + butyrate | - |
? | |
triolein + H2O | - |
Penaeus vannamei | ? | - |
? | |
tripalmitin + H2O | - |
Penaeus vannamei | ? | - |
? | |
tristearin + H2O | - |
Penaeus vannamei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 95260 + 1 * 63360, calculated from SDS-PAGE | Penaeus vannamei |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Penaeus vannamei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Penaeus vannamei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | at 20°C and at 50°C, about 50% of the activity is lost and at 60°C, activity falls abruptly by denaturation | Penaeus vannamei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200.2 | - |
4-Methylumbelliferyl butyrate | apparent value, in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Penaeus vannamei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Penaeus vannamei | isoelectric focusing | - |
5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1 | - |
in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei | diethyl 4-nitrophenyl phosphate |
General Information | Comment | Organism |
---|---|---|
physiological function | the lipase has a potential role in hydrolysis of triacylglycerides stored as fat body | Penaeus vannamei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.88 | - |
4-Methylumbelliferyl butyrate | apparent value, in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei |