Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Penicillium expansum |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using ammonia sulfate as the precipitant at 25°C | Penicillium expansum |
Protein Variants | Comment | Organism |
---|---|---|
K202A | the mutation takes place near the active site, and may thus perturb the active site conformation | Penicillium expansum |
additional information | the active site of the lipase is identified to be S132, D188, and H241 | Penicillium expansum |
P163A | the mutation takes place near the active site, and may thus perturb the active site conformation | Penicillium expansum |
R182K | the mutation takes place near the active site, and may thus perturb the active site conformation | Penicillium expansum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium expansum | Q7LST4 | - |
- |
Penicillium expansum PF898 | Q7LST4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Penicillium expansum |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Penicillium expansum |