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Literature summary for 3.1.1.3 extracted from
- Purification, physico-chemical and kinetic properties of the deglycosylated Talaromyces thermophilus lipase (2012), Int. J. Biol. Macromol., 51, 892-900 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces dupontii | F6LQK7 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | treatment with endoglycosidase H decreases molecular mass from 39000 Da to 30000 Da | Thermomyces dupontii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomyces dupontii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2100 | - |
deglycosylated form, substrate glyceryl tributanoate, 45°C, pH 9.5 | Thermomyces dupontii |
| 4642 | - |
deglycosylated form, substrate olive oil emulsion, 45°C, pH 9.5 | Thermomyces dupontii |
| 9608 | - |
glycosylated form, substrate olive oil emulsion, 50°C, pH 9.5 | Thermomyces dupontii |
| 12000 | - |
deglycosylated form, substrate glyceryl trioctanoate, 45°C, pH 9.5 | Thermomyces dupontii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyceryl tributanoate + H2O | - |
Thermomyces dupontii | glycerol + butanoate | - |
? |  |
| glyceryl trioctanoate + H2O | - |
Thermomyces dupontii | glycerol + octanoate | - |
? | |
| olive oil + H2O | - |
Thermomyces dupontii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, deglycosylated form, x * 39000, glycosylated form, SDS-PAGE, x * 29590, calculated | Thermomyces dupontii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TTL | - |
Thermomyces dupontii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
deglycosylated form | Thermomyces dupontii |
| 50 | - |
glycosylated form | Thermomyces dupontii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Thermomyces dupontii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | deglycosylation of the enzyme reduces its specific activity by 50% | Thermomyces dupontii |
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