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Literature summary for 3.1.1.32 extracted from

  • Richmond, G.S.; Smith, T.K.
    The role and characterization of phospholipase A1 in mediating lysophosphatidylcholine synthesis in Trypanosoma brucei (2007), Biochem. J., 405, 319-329.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information TbPLA1 does not require metal cofactors for activity, but it does require interfacial activation prior to catalysis, activation by Triton X-100/glycerophosphatidylcholine mixed micelle surfaces is not specific and does not require the preformation of a specific enzyme-substrate complex to achieve surface binding, activation kinetics and mechanism, overview Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information construction of the PLA1 deletion mutant cell line BSF lacking enzyme activity, overview Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
arachidonyl trifluoromethyl ketone analogue
-
Trypanosoma brucei
Ca2+ slight inhibition at 2 mM Trypanosoma brucei
Cd2+ complete inhibition at 2 mM Trypanosoma brucei
Co2+ 43% inhibition at 2 mM Trypanosoma brucei
Cu2+ complete inhibition at 2 mM Trypanosoma brucei
di-isopropyl fluorophosphate TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei
diethyl-p-nitrophenyl phosphate
-
Trypanosoma brucei
E-600
-
Trypanosoma brucei
Fe2+ complete inhibition at 2 mM Trypanosoma brucei
Mn2+ slight inhibition at 2 mM Trypanosoma brucei
additional information PLA1 is not affected by Mg2+, Cs2+, and EDTA Trypanosoma brucei
Ni2+ 57% inhibition at 2 mM Trypanosoma brucei
palmitoyl trifluoromethyl ketone analogue
-
Trypanosoma brucei
PMSF TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei
Zn2+ 66% inhibition at 2 mM Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic analysis of enzyme activation Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
additional information TbPLA1 does not require metal co-factors for activity Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29700
-
soluble free enzyme, gel filtration Trypanosoma brucei
137000
-
Triton X-100 micelle-bound PLA1, gel filtration Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Trypanosoma brucei
-
lysophosphocholine + a carboxylate
-
?
phosphatidylcholine + H2O Trypanosoma brucei 427
-
lysophosphocholine + a carboxylate
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q6KBS7 from BALB/c mouse bloodstream
-
Trypanosoma brucei 427 Q6KBS7 from BALB/c mouse bloodstream
-

Source Tissue

Source Tissue Comment Organism Textmining
trypomastigote from insect and bloodstream, the latter enzyme form shows a 3fold higher activity Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 8 recombinant enzyme Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
Trypanosoma brucei lysophosphocholine + a carboxylate
-
?
phosphatidylcholine + H2O TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei lysophosphocholine + a carboxylate
-
?
phosphatidylcholine + H2O
-
Trypanosoma brucei 427 lysophosphocholine + a carboxylate
-
?
phosphatidylcholine + H2O TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei 427 lysophosphocholine + a carboxylate
-
?

Synonyms

Synonyms Comment Organism
PLA1
-
Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Trypanosoma brucei