Literature summary for 3.1.1.32 extracted from

Kunduri, G.; Yuan, C.; Parthibane, V.; Nyswaner, K.; Kanwar, R.; Nagashima, K.; Britt, S.; Mehta, N.; Kotu, V.; Porterfield, M.; Tiemeyer, M.; Dolph, P.; Acharya, U.; Acharya, J.
Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors (2014), J. Cell Biol., 206, 79-96.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene CG8552, expression of a 31-kb genomic enzyme fragment modified to carry a V5 epitope at the N-terminus, i.e. V5-N-PAPLA1, the recombinant protein is transiently expressed and fully functional in the Drosophila S2 cell line and rescues all the phenotype of a null mutant	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a gene CG8552 null mutant	Drosophila melanogaster

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Drosophila melanogaster	5829	-

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	M9MRD9	isozyme b; gene CG8552 or papla1	-
Drosophila melanogaster	Q9VLS7	isozyme a; gene CG8552 or papla1	-
Drosophila melanogaster	X2JDJ3	isozyme D; gene CG8552 or papla1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
head	-	Drosophila melanogaster	-
male accessory gland	-	Drosophila melanogaster	-
additional information	the enzyme protein shows strong colocalization with the Sec23 protein in a number of tissues, including oocyte and male accessory gland	Drosophila melanogaster	-
oocyte	-	Drosophila melanogaster	-

Synonyms

Synonyms	Comment	Organism
PAPLA1	-	Drosophila melanogaster
phosphatidic acid phospholipase A1	-	Drosophila melanogaster

General Information

General Information	Comment	Organism
malfunction	in enzyme mutants, in the absence of transport to the Golgi, rhodopsin 1 is aberrantly glycosylated and is mislocalized. These defects lead to decreased levels of the protein and decreased sensitivity of the photoreceptors to light. Several GPCRs, including other rhodopsins and Bride of sevenless, are similarly affected, phenotypes, overview	Drosophila melanogaster
physiological function	the cytosolic enzyme is necessary for transit of selective transmembrane receptor cargo by the COPII coat for anterograde trafficking in Drosophila melanogaster. The enzme interacts with the coat protein II (COPII), the COPII coated vesicular system transports newly synthesized secretory and membrane proteins from the endoplasmic reticulum to the Golgi complex. The enzyme is required for the transport of rhodopsin 1, an N-glycosylated G protein-coupled receptor, from the endoplasmic reticulum to the Golgi complex, but the active site of PAPLA1 is not necessary for Rh1 maturation. The enzyme acts downstream of the rhodopsin 1 chaperone NinaA and upstream of the Golgi-resident protein dMPPE	Drosophila melanogaster

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Release 2023.1 (January 2023)