Application | Comment | Organism |
---|---|---|
synthesis | enantiomerically enriched diarylmethanols are useful as the precursors or building blocks for the synthesis of pharmaceutically important compounds such as antihistaminic, antiarrhythmic, and anticholinergic agents. Successful application of this LPL preparation (LPL-D1) to the dynamic kinetic resolution (DKR) of diarylmethanols including aryl heteroarylmethanols, method, overview | Burkholderia sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme coated with both dextrin (D) and ionic surfactant (i) via lyophilization, LPL-D1, is prepared by freeze-drying a solution containing LPL (52% protein), dextrin (D), and surfactant at a 1:2:1 weight ratio in 1:1 (v/v) water-dioxane. LPL-D1 is as active as its native counterpart in water but about 3000fold more active than the latter in organic solvent | Burkholderia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
triacylglycerol + H2O | Burkholderia sp. | - |
diacylglycerol + a carboxylate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | lipoprotein lipase (LPL) from Burkholderia species is also highly active in organic solvent if it is coated with both dextrin (D) and ionic surfactant (i) via lyophilization. LPL-D1 is as active as its native counterpart in water but about 3000fold more active than the latter in organic solvent | Burkholderia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | lipoprotein lipase (LPL) from Burkholderia species is also highly active in organic solvent if it is coated with both dextrin (D) and ionic surfactant (i) via lyophilization. Successful application of this LPL preparation (LPL-D1) to the dynamic kinetic resolution (DKR) of diarylmethanols including aryl heteroarylmethanols. Enzyme assay performing hydrolysis and alcoholysis of 4-nitrophenyl acetate. The enantioselectivity of LPL-D1 increases with increasing difference in size between two aryl groups at the hydroxymethine center of substrate. The enantioselectivity is low for monohalogenated phenyl acetate but increases significantly in the case that the halogen is substituted by a bulkier substituent such as isopropyl, tert-butyl or trimethylsilyl | Burkholderia sp. | ? | - |
? | |
triacylglycerol + H2O | - |
Burkholderia sp. | diacylglycerol + a carboxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LPL-D1 | - |
Burkholderia sp. |
More | cf. EC 3.1.1.6 | Burkholderia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | assay at | Burkholderia sp. |