Any feedback?
Literature summary for 3.1.1.4 extracted from
- Expression, purification and kinetic characterization of a recombinant 80-kDa intracellular calcium-independent phospholipase A2 (1996), J. Biol. Chem., 271, 30879-30885.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mesocricetus auratus |  |
| Calmodulin | - |
Mesocricetus auratus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pET21a vector for transformation of Escherichia coli strain BL21(DE3) and into pFasBac baculovirus-vector for transfection of Sf9 S. frugiperda cells | Mesocricetus auratus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (E)-6-(bromomethylene)-3-(1-naphthalenyl)-2H-tetrahydropyran-2-one | - |
Mesocricetus auratus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
1-palmitoyl-2-arachidonylphosphatidylcholine | PLA2 activity | Mesocricetus auratus | |
| 0.0033 | - |
1-palmitoyl-2-arachidonylphosphatidylcholine | PLA1- and lysophospholipase activity of the 80-kDa PLA2 | Mesocricetus auratus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mesocricetus auratus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
- |
Mesocricetus auratus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Mesocricetus auratus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| tandem affinity chromatographies | Mesocricetus auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ovary | - |
Mesocricetus auratus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | - |
substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine | Mesocricetus auratus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-hexadecyl-2-arachidonyl-glycero-3-phosphocholine + H2O | - |
Mesocricetus auratus | 1-O-hexadecyl-glycero-3-phosphocholine + arachidonic acid | - |
? | |
| 1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O | substrate for measurement of PLA1-activity of the 80-kDa PLA2 | Mesocricetus auratus | 1-palmitoyl-glycerophosphorylcholine + arachidonic acid | - |
? | |
| phospholipids + H2O | - |
Mesocricetus auratus | ? | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
