Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Oryctolagus cuniculus | |
EDTA | - |
Oryctolagus cuniculus | |
iodoacetamide | weak inhibitor | Oryctolagus cuniculus | |
N-ethylmaleimide | weak inhibitor | Oryctolagus cuniculus | |
p-bromophenacyl bromide | - |
Oryctolagus cuniculus | |
p-hydroxymercuribenzoate | weak inhibitor | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
1-palmitoyl-2-arachidonoylphosphatidylethanolamine | at pH 8.5, without detergents | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Oryctolagus cuniculus | 5764 | - |
microsome | - |
Oryctolagus cuniculus | - |
- |
mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Oryctolagus cuniculus | |
Cd2+ | 1% of the activity obtained with Ca2+ | Oryctolagus cuniculus | |
Sr2+ | 1% of the activity obtained with Ca2+ | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6500 | - |
gel filtration, underestimation seems obvious to authors | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipids + H2O | Oryctolagus cuniculus | inflammatory diseases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion-exchange chromatography, gel-filtration chromatography, hydroxyapatite chromatography, 72500fold | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
290 | - |
substrate: 1-palmitoyl-2-oleolylphosphatidylcholine | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, in buffer A, 71 days, 4% decrease of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dipalmitoyl-phosphatidylcholine + H2O | - |
Oryctolagus cuniculus | 1-palmitoyl-glycerophosphorylcholine + palmitic acid | - |
? | |
1-palmitoyl-2-arachidonoylphosphatidylethanolamine | - |
Oryctolagus cuniculus | 1-palmitoyl-glycerophosphorylethanolamine + arachidonic acid | - |
? | |
1-palmitoyl-2-oleoylphosphatidylcholine + H2O | - |
Oryctolagus cuniculus | 1-palmitoyl-glycerophosphocholine + oleic acid | - |
? | |
phospholipids + H2O | inflammatory diseases | Oryctolagus cuniculus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
substrate: phosphatidylcholine | Oryctolagus cuniculus |
9.5 | 10 | substrate: phosphatidylethanolamine | Oryctolagus cuniculus |