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Literature summary for 3.1.1.4 extracted from

  • Deems, R.A.; Dennis, E.A.
    Phospholipase A2 from cobra venom (Naja naja naja) (1981), Methods Enzymol., 71, 703-710.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine activates hydrolysis of phosphatidylethanolamine Naja naja
sphingomyelin activates hydrolysis of phosphatidylethanolamine Naja naja

General Stability

General Stability Organism
guanidine-HCL, PLA2 retains 60% of activity when assay contains 6 M of guanidine-HCl Naja naja

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Naja naja
p-bromophenacyl bromide
-
Naja naja
Sr2+ competitive inhibition Naja naja

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 5 phosphatidylcholine Triton X-100/phospholipid: 2/1 Naja naja

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Naja naja

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
gel filtration, SDS-PAGE, analytical ultracentrifugation Naja naja

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Naja naja
-
lysophosphatidylcholine + a fatty acid
-
?
phospholipids + H2O Naja naja
-
?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone PLA2 regains almost full activity when added to assay mixture Naja naja
benzene PLA2 regains almost full activity when added to assay mixture Naja naja
chloroform PLA2 regains almost full activity when added to assay mixture Naja naja
ether PLA2 regains almost full activity when added to assay mixture Naja naja

Organism

Organism UniProt Comment Textmining
Naja naja
-
cobra
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography, 15fold Naja naja

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1129
-
substrate: 1,2-diacyl-phosphatidylcholine Naja naja
2000
-
Triton X-100/phospholipid: 2/1 Naja naja

Storage Stability

Storage Stability Organism
frozen, PLA2 concentration below 2 mg/ml, no loss of activity or precipitation for up to 12 months Naja naja

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-diacyl-sn-glycero-3-phosphorylcholine + H2O
-
Naja naja 1-acyl-sn-glycero-3-phosphorylcholine + fatty acid
-
?
phosphatidylcholine + H2O
-
Naja naja lysophosphatidylcholine + a fatty acid
-
?
phosphatidylethanolamine + H2O
-
Naja naja 1-acylglycerophosphorylethanolamine + fatty acid
-
?
phosphatidylserine + H2O
-
Naja naja 1-acylglycerophosphoserine + fatty acid
-
?
phospholipids + H2O
-
Naja naja ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, dimerization occurs at enzyme concentrations between 0.1 and 2 mg PLA2 per ml Naja naja
monomer monomeric when enzyme concentration is below 0.05 mg PLA2 per ml, 13000, gel filtration, SDS-PAGE, analytical ultracentifugation Naja naja
polymer 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, polimerization at enzyme concentrations above 5mg PLA2 per ml Naja naja

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
for 10 min at pH 3-4, only 5% loss in activity Naja naja

pH Range

pH Minimum pH Maximum Comment Organism
7 9 substrate: 1,2-diacyl-phosphatidylcholine Naja naja