Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | activates hydrolysis of phosphatidylethanolamine | Naja naja | |
sphingomyelin | activates hydrolysis of phosphatidylethanolamine | Naja naja |
General Stability | Organism |
---|---|
guanidine-HCL, PLA2 retains 60% of activity when assay contains 6 M of guanidine-HCl | Naja naja |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Naja naja | |
p-bromophenacyl bromide | - |
Naja naja | |
Sr2+ | competitive inhibition | Naja naja |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 5 | phosphatidylcholine | Triton X-100/phospholipid: 2/1 | Naja naja |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Naja naja |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
gel filtration, SDS-PAGE, analytical ultracentrifugation | Naja naja |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Naja naja | - |
lysophosphatidylcholine + a fatty acid | - |
? | |
phospholipids + H2O | Naja naja | - |
? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | PLA2 regains almost full activity when added to assay mixture | Naja naja |
benzene | PLA2 regains almost full activity when added to assay mixture | Naja naja |
chloroform | PLA2 regains almost full activity when added to assay mixture | Naja naja |
ether | PLA2 regains almost full activity when added to assay mixture | Naja naja |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Naja naja | - |
cobra | - |
Purification (Comment) | Organism |
---|---|
column chromatography, 15fold | Naja naja |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1129 | - |
substrate: 1,2-diacyl-phosphatidylcholine | Naja naja |
2000 | - |
Triton X-100/phospholipid: 2/1 | Naja naja |
Storage Stability | Organism |
---|---|
frozen, PLA2 concentration below 2 mg/ml, no loss of activity or precipitation for up to 12 months | Naja naja |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diacyl-sn-glycero-3-phosphorylcholine + H2O | - |
Naja naja | 1-acyl-sn-glycero-3-phosphorylcholine + fatty acid | - |
? | |
phosphatidylcholine + H2O | - |
Naja naja | lysophosphatidylcholine + a fatty acid | - |
? | |
phosphatidylethanolamine + H2O | - |
Naja naja | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
phosphatidylserine + H2O | - |
Naja naja | 1-acylglycerophosphoserine + fatty acid | - |
? | |
phospholipids + H2O | - |
Naja naja | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, dimerization occurs at enzyme concentrations between 0.1 and 2 mg PLA2 per ml | Naja naja |
monomer | monomeric when enzyme concentration is below 0.05 mg PLA2 per ml, 13000, gel filtration, SDS-PAGE, analytical ultracentifugation | Naja naja |
polymer | 13000, identical subunits, gel filtration, SDS-PAGE, analytical ultracentifugation, polimerization at enzyme concentrations above 5mg PLA2 per ml | Naja naja |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
for 10 min at pH 3-4, only 5% loss in activity | Naja naja |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | substrate: 1,2-diacyl-phosphatidylcholine | Naja naja |