Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, residual activity 82.1% | Aeropyrum pernix | |
KCl | 1 mM, residual activity 78.9% | Aeropyrum pernix | |
MgCl2 | 1 mM, residual activity 71.6% | Aeropyrum pernix | |
additional information | not inhibitory: EDTA | Aeropyrum pernix | |
NaCl | 1 mM, residual activity 58.5% | Aeropyrum pernix | |
ZnCl2 | 1 mM, no residual activity | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.103 | - |
p-nitrophenyl propionate | 90°C, pH 8.0 | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for Ca2+ | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, SDS-PAGE | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
var. K1, bifunctional enzyme bwith both phospholipase A2 and esterase activity | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
249 | - |
90°C, pH 8.0 | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: p-nitrophenyl palmitate, p-nitrophenyl stearate | Aeropyrum pernix | ? | - |
? | |
p-nitrophenyl laurate + H2O | 23.7% of the activity with p-nitrophenyl propionate | Aeropyrum pernix | p-nitrophenol + laurate | - |
? | |
p-nitrophenyl octanoate + H2O | 79.5% of the activity with p-nitrophenyl propionate | Aeropyrum pernix | p-nitrophenol + octanoic acid | - |
? | |
p-nitrophenyl propionate + H2O | - |
Aeropyrum pernix | p-nitrophenol + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, SDS-PAGE | Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life of about 1 h | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
39 | - |
p-nitrophenyl propionate | 90°C, pH 8.0 | Aeropyrum pernix |