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Literature summary for 3.1.1.4 extracted from

  • Santos, K.F.; Murakami, M.T.; Cintra, A.C.; Toyama, M.H.; Marangoni, S.; Forrer, V.P.; Brandao Neto, J.R.; Polikarpov, I.; Arni, R.K.
    Crystallization and preliminary X-ray crystallographic analysis of the heterodimeric crotoxin complex and the isolated subunits crotapotin and phospholipase A2 (2007), Acta Crystallogr. Sect. F, 63, 287-290.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
purified crotoxin, 10 mg/ml protein in 20 mM Tris–HCl buffer, pH 8.0, hanging-drop vapour-diffusion method, 0.001 ml of protein and of reservoir solution are mixed and equilibrated against 1 ml of reservoir solution containing 0.1 M Tris-HCl pH 8.5 and 8% w/v PEG 8000 within 17 days, or of reservoir solution containing 0.05 M cadmium sulfate, 0.1 M HEPES, pH 7.5, and 1 M sodium acetate within within 30 days, or of reservoir solution containing 28% v/v PEG 400, 0.1 M HEPES pH 7.5, 0.2 M calcium chloride dehydrate and 3-9% v/v glycerol, X-ray diffraction structure determination and analysis at 1.75-2.6 A resolution Crotalus durissus terrificus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9490
-
1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit Crotalus durissus terrificus
14350
-
1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit Crotalus durissus terrificus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Crotalus durissus terrificus crotoxin, a potent neurotoxin from the venom of the Brazilian rattlesnake, exists as a heterodimer formed between a phospholipase A2 and a catalytically inactive acidic phospholipase A2 analogue crotapotin, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Crotalus durissus terrificus
-
South American rattlesnake
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Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Crotalus durissus terrificus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information crotoxin, a potent neurotoxin from the venom of the Brazilian rattlesnake, exists as a heterodimer formed between a phospholipase A2 and a catalytically inactive acidic phospholipase A2 analogue crotapotin, overview Crotalus durissus terrificus ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 14350, phospholipase A2 subunit, + 1 * 9490, crotapin subunit Crotalus durissus terrificus
More crotoxin, a potent neurotoxin from the venom of the South American rattlesnake, exists as a heterodimer formed between a phospholipase A2 and a catalytically inactive acidic phospholipase A2 analogue crotapotin, crotapotin consists of three polypeptide chains linked together by disulfide bridges and acts as an inhibitory chaperone subunit that prevents promiscuous interactions of the phospholipase with phospholipids on membrane surfaces other than its target membrane, overview Crotalus durissus terrificus

Synonyms

Synonyms Comment Organism
crotoxin
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Crotalus durissus terrificus
PLA2
-
Crotalus durissus terrificus

pI Value

Organism Comment pI Value Maximum pI Value
Crotalus durissus terrificus crotapin subunit, sequence calculation
-
3.4
Crotalus durissus terrificus phospholipase A2 subunit, sequence calculation
-
8.2