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Literature summary for 3.1.1.4 extracted from
- Purification and biochemical characterization of phospholipase A2 from dromedary pancreas (2006), Biochim. Biophys. Acta, 1760, 1202-1209.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium taurodeoxycholate | activates maximally at 3 mM | Camelus dromedarius |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates maximally at 7 mM CaCl2 | Camelus dromedarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13749 | - |
1 * 13749 | Camelus dromedarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Camelus dromedarius | - |
1-acylglycerophosphocholine + a carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Camelus dromedarius |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 2000fold from delipidated pancreas by heat and acid treatment at 70°C and pH 3.0, ammonium sulfate fractionation, ethanol precipitation, gel filtration, anion and cation exchange chromatography, and reversed phase chromatography | Camelus dromedarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 600 | - |
purified enzyme | Camelus dromedarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Camelus dromedarius | 1-acylglycerophosphocholine + a carboxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 13749 | Camelus dromedarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DrPLA2 | - |
Camelus dromedarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Camelus dromedarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | temperature profile, overview | Camelus dromedarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Camelus dromedarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH profile, overview | Camelus dromedarius |
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