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Literature summary for 3.1.1.4 extracted from
- Ostrich pancreatic phospholipase A2: purification and biochemical characterization (2007), J. Chromatogr. B, 857, 108-114.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for activity | Struthio camelus |  |
| sodium taurodeoxycholate | activates | Struthio camelus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
pancreatic PLA2, gel filtration | Struthio camelus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Struthio camelus | - |
1-acylglycerophosphocholine + a carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Struthio camelus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 280fold from delipidated pancreas by heat treatment at 70°C, ammonium sulfate and ethanol fractionation, anion exchange chromatography, and gel filtration to homogeneity | Struthio camelus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Struthio camelus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 840 | - |
purified enzyme | Struthio camelus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Struthio camelus | 1-acylglycerophosphocholine + a carboxylate | - |
? | |
| phosphatidylcholine + H2O | substrate is derived from egg yolk | Struthio camelus | 1-acylglycerophosphocholine + a carboxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 13773.93, pancreatic PLA2, mass spectrometry | Struthio camelus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OPLA2 | - |
Struthio camelus |
| pancreatic phospholipase A2 | - |
Struthio camelus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 40 | - |
Struthio camelus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 55 | activity range | Struthio camelus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
20 min, purified pancreatic PLA2, over 80% remaining activity, 70% reamining activity after 1.5 h | Struthio camelus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Struthio camelus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 11 | 10 min, purified pancreatic PLA2, stable | Struthio camelus |
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Release 2023.1 (January 2023)
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