Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged and/or fluoresence-tagged phospholipase A2 in COS-7 cells, perinuclear region, membranes and cytosol, or 3T3-L1 preadipocytic cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7,7-dimethyl-5,8-eicosadienoic acid | - |
Rattus norvegicus | |
arachidonyl trifluoromethyl ketone | i.e. AACOCF3 | Rattus norvegicus | |
methyl arachidonyl fluorophosphate | i.e. MAFP, an inhibitor of cysteine-dependent phospholipases | Rattus norvegicus | |
additional information | no inhibition of AdPLA and sPLA2 by bromoenolactone | Rattus norvegicus | |
thioetheramide | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
1-palmitoyl-2-linoleoyl-phosphatidylethanolamine | pH 8.0, 25°C, recombinant enzyme | Rattus norvegicus | |
0.0073 | - |
1-palmitoyl-2-linoleoyl-phosphatidylserine | pH 8.0, 25°C, recombinant enzyme | Rattus norvegicus | |
0.0082 | - |
1-palmitoyl-2-arachidonyl-phosphatidylcholine | pH 8.0, 25°C, recombinant enzyme | Rattus norvegicus | |
0.0116 | - |
1-palmitoyl-2-linoleoyl-phosphatidic acid | pH 8.0, 25°C, recombinant enzyme | Rattus norvegicus | |
0.0175 | - |
1,2-dilinoleoyl-phosphatidylcholine | pH 8.0, 25°C, recombinant enzyme | Rattus norvegicus | |
0.0186 | - |
1-palmitoyl-2-linoleoyl-phosphatidylcholine | pH 8.0, 25°C, recombinant enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | AdPLA | Rattus norvegicus | 5829 | - |
membrane | the adipose-specific phospholipase A2 AdPLA is membrane-associated | Rattus norvegicus | 16020 | - |
additional information | subcellular localization of AdPLA, overview | Rattus norvegicus | - |
- |
perinuclear space | AdPLA | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the adipose-specific phospholipase A2 is activated by Ca2+, but it is not essential for activity | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female Zucker rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon | - |
Rattus norvegicus | - |
gonad | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
additional information | expression profiles of isozymes iPLA2beta, sPLA2alpha, and AdPLA, overview | Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
skin | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
white adipose tissue | AdPLA is highly expressed specifically in white adipose tissue and is induced during preadipocyte differentiation into adipocytes, overview | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dilinoleoyl-phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-linoleoyl-sn-glycero-3-phosphocholine + linoleic acid | - |
? | |
1,2-dilinoleoyl-phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-linoleoyl-glycerophosphorylcholine + linoleic acid | - |
? | |
1-palmitoyl-2-arachidonyl-phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonic acid | - |
? | |
1-palmitoyl-2-linoleoyl-phosphatidic acid + H2O | - |
Rattus norvegicus | 1-palmitoyl-sn-glycero-3-phosphate + linoleic acid | - |
? | |
1-palmitoyl-2-linoleoyl-phosphatidylcholine + H2O | - |
Rattus norvegicus | 1-palmitoyl-sn-glycero-3-phosphocholine + linoleic acid | - |
? | |
1-palmitoyl-2-linoleoyl-phosphatidylethanolamine + H2O | - |
Rattus norvegicus | 1-palmitoyl-sn-glycerophosphoethanolamine + linoleic acid | - |
? | |
1-palmitoyl-2-linoleoyl-phosphatidylinositol + H2O | - |
Rattus norvegicus | 1-palmitoyl-3-phosphoinositol + linoleic acid | - |
? | |
1-palmitoyl-2-linoleoyl-phosphatidylserine + H2O | - |
Rattus norvegicus | 1-palmitoyl-sn-glycero-3-phosphoserine + linoleic acid | - |
? | |
hen egg phosphatidylcholine + H2O | - |
Rattus norvegicus | ? | - |
? | |
additional information | phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids | Rattus norvegicus | ? | - |
? | |
additional information | AdPLA is most likely a His-Cys PLA2, His23 and Cys113 are critical for activity of AdPLA, catalytic mechanism, overview. AdPLA has phospholipase activity but lacks acyltransferase activity | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | AdPLA contains no characteristic serine lipase motif, i.e. GXSXG or GXSGS | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
AdPLA | - |
Rattus norvegicus |
cPLA2alpha | - |
Rattus norvegicus |
specific phospholipase A2 | - |
Rattus norvegicus |
sPLA2 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |