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Literature summary for 3.1.1.4 extracted from
- A novel hepatopancreatic phospholipase A2 from Hexaplex trunculus with digestive and toxic activities (2010), Arch. Biochem. Biophys., 494, 121-129.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | slight increase in phospholipase activity during the summer | Hexaplex trunculus | |
| sodium deoxycholate | is required for activity, maximum activity above 4 mM | Hexaplex trunculus |  |
| sodium taurodeoxycholate | is required for activity, maximum activity above 4 mM | Hexaplex trunculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Hexaplex trunculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | specific activity increases to reach its maximum in the presence of 10 mM Ca2+ using phosphatidylcholine as substrate | Hexaplex trunculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration | Hexaplex trunculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | - |
- |
- |
| Hexaplex trunculus | - |
- |
- |
| Struthio camelus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Camelus dromedarius |
- |
Struthio camelus |
| by ammonium sulfate and ethanol precipitation and gel filtration, to homogeneity, 250fold. Overall recovery of phospholipase activity is about 82% | Hexaplex trunculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Hexaplex trunculus | - |
| pancreas | - |
Sus scrofa | - |
| pancreas | - |
Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.72 | - |
crude extract, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2 | Hexaplex trunculus |
| 180 | - |
250fold purified enzyme, at 50°C and pH 8.5, with phosphatidylcholine liposomes as a substrate in the presence of 4 mM sodium taurodeoxycholate and 6 mM CaCl2 | Hexaplex trunculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-stearoyl-2-oleoyl-3-sn-glycerophosphorylcholine + H2O | - |
Hexaplex trunculus | ? | - |
? | |
| egg yolk + H2O | - |
Hexaplex trunculus | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Hexaplex trunculus | 1-acylglycerophosphocholine + fatty acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Hexaplex trunculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| marine snail digestive phospholipase A2 | - |
Hexaplex trunculus |
| mSDPL | - |
Hexaplex trunculus |
| phospholipase A2 | - |
Sus scrofa |
| phospholipase A2 | - |
Camelus dromedarius |
| phospholipase A2 | - |
Struthio camelus |
| phospholipase A2 | - |
Hexaplex trunculus |
| PLA2 | - |
Sus scrofa |
| PLA2 | - |
Camelus dromedarius |
| PLA2 | - |
Struthio camelus |
| PLA2 | - |
Hexaplex trunculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Hexaplex trunculus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | - |
Hexaplex trunculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
retains more than 80% or 40% of its initial activity after 30 min of incubation at 40 or 50°C, respectively. The enzyme is completely inactivated after incubation at 55°C during 30 min. Presence of substrate increases thermostability | Hexaplex trunculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Hexaplex trunculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Hexaplex trunculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | is highly stable over a broad pH range, maintains over 100% of its maximal activity between pH 7.0 and 9.0 and 60% at pH 10. The enzyme is completely inactivated at pH 4.0 for 30 min | Hexaplex trunculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | does not show any hemolysis activity in intact erythrocytes | Sus scrofa |
| physiological function | does not show any hemolysis activity in intact erythrocytes. Is active at low surface pressure | Camelus dromedarius |
| physiological function | does not show any hemolysis activity in intact erythrocytes. Is active at low surface pressure | Struthio camelus |
| physiological function | exhibits hemolytic activity in intact erythrocytes and can penetrate phospholipid monolayers at high surface pressure. May be toxic to mammal cells | Hexaplex trunculus |
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