Literature summary for 3.1.1.4 extracted from

Lee, C.W.; Lee, I.T.; Lin, C.C.; Lee, H.C.; Lin, W.N.; Yang, C.M.
Activation and induction of cytosolic phospholipase A2 by IL-1beta in human tracheal smooth muscle cells: role of MAPKs/p300 and NF-kappaB (2010), J. Cell. Biochem., 109, 1045-1056.

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Activating Compound

Activating Compound	Comment	Organism	Structure
IL-1beta	stimulates transient phosphorylation of cPLA2 in a time-dependent manner	Homo sapiens

Application

Application	Comment	Organism
drug development	the mechanisms by which IL-1beta-induces cPLA2 expression may be an important link in the pathogenesis of airway inflammatory diseases. Understanding these mechanisms underlying IL-1beta-induced cPLA2 expression in human tracheal smooth muscle cells is important to develop new therapeutic strategies	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
for construction of the cPLA2-luc plasmid, cPLA2 promoter, a region spanning -2,375 to -75 bp, cloned into pGL3-basic vector	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
AACOCF3	significantly inhibits IL-1beta-stimulated prostaglandin E2 synthesis	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
tracheal smooth muscle cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
cPLA2	-	Homo sapiens
cytosolic phospholipase A2	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	IL-1beta-mediated induction of cPLA2 expression is abolished by either actinomycin D or cycloheximide in a concentration-dependent manner. SB202190 or SP600125 inhibit cPLA2 protein and mRNA expression induced by IL-1beta. Transfection with JNK2 siRNA or p300 siRNA blocks cPLA2 expression induced by IL-1beta. AACOCF3, U0126, SB202190, SP600125, or helenalin significantly inhibit IL-1beta-stimulated prostaglandin E2 synthesis linked to cPLA2 activity	down
Homo sapiens	IL-1beta induces cPLA2 protein expression in a time-dependent manner with a maximal response within 16-24 h. IL-1beta also enhances cPLA2 mRNA accumulation in a time-dependent manner with a maximal response within 5 h. IL-1beta-induced cPLA2 expression requires ongoing transcription and translation. Association of p300 and histone H4 leads to cPLA2 gene transcription. In human tracheal smooth muscle cells, activation of MAPKs (p42/p44 MAPK and p38 MAPK), NF-kappaB, JNK1/2 an the co-activator p300 are essential for IL-1beta-induced cPLA2 expression and prostaglandin E2 secretion	up

General Information

General Information	Comment	Organism
physiological function	IL-1beta-induced prostaglandin E2 generation is dependent on the up-regulation of cPLA2 expression in tracheal smooth muscle cells	Homo sapiens

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Release 2023.1 (January 2023)