Application | Comment | Organism |
---|---|---|
medicine | PLA2 displays an inhibitory effect, independent from its catalytic activity, on tumor cell adhesion and migration, which is mediated via specific inhibition of integrins | Macrovipera lebetina transmediterranea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-bromophenacyl bromide | abolishes the enzymatic activity of PLA2 without affecting its anti-tumor effect, suggesting the presence of pharmacological sites distinct from the catalytic site | Macrovipera lebetina transmediterranea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13627 | - |
x * 13627, mass spectrometry. x * 13638, calculated from sequence | Macrovipera lebetina transmediterranea |
13638 | - |
x * 13627, mass spectrometry. x * 13638, calculated from sequence | Macrovipera lebetina transmediterranea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrovipera lebetina transmediterranea | B5U6Z2 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by gel filtration and reverse-phase chromatography | Macrovipera lebetina transmediterranea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | seems to be the unique PLA2 in this venom | Macrovipera lebetina transmediterranea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63.51 | - |
at 37°C, pH 7 | Macrovipera lebetina transmediterranea |
Subunits | Comment | Organism |
---|---|---|
? | x * 13627, mass spectrometry. x * 13638, calculated from sequence | Macrovipera lebetina transmediterranea |
Synonyms | Comment | Organism |
---|---|---|
acidic Asp49 phospholipase A2 | - |
Macrovipera lebetina transmediterranea |
group II snake venom phospholipase A2 | - |
Macrovipera lebetina transmediterranea |
svPLA2 | - |
Macrovipera lebetina transmediterranea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Macrovipera lebetina transmediterranea | calculated from sequence | - |
4.69 |
General Information | Comment | Organism |
---|---|---|
physiological function | PLA2 inhibits efficiently tumor cells adhesion and migration. PLA2 is not cytotoxic up to 0.002 mM during 4 days on human cell lines IGR39 (melanoma) and HT1080 (fibrosarcoma). Completely abolishes cell adhesion of IGR39 cells to fibrinogen and fibronectin in a dose-dependent manner, while attachment to laminin-1 and vitronectin is only slightly affected. The enzyme has no effect on type I collagen, integrin-independent substratum and poly-L-lysine. PLA2 abolishes HT1080 cells migration towards fibronectin and fibrinogen in a dose-dependent manner. Anti-tumor effect of PLA2 is mediated by alpha5beta1 and alphav-containing integrins | Macrovipera lebetina transmediterranea |