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Literature summary for 3.1.1.4 extracted from

  • Bazaa, A.; Luis, J.; Srairi-Abid, N.; Kallech-Ziri, O.; Kessentini-Zouari, R.; Defilles, C.; Lissitzky, J.C.; El Ayeb, M.; Marrakchi, N.
    MVL-PLA2, a phospholipase A2 from Macrovipera lebetina transmediterranea venom, inhibits tumor cells adhesion and migration (2009), Matrix Biol., 28, 188-193.
    View publication on PubMed

Application

Application Comment Organism
medicine PLA2 displays an inhibitory effect, independent from its catalytic activity, on tumor cell adhesion and migration, which is mediated via specific inhibition of integrins Macrovipera lebetina transmediterranea

Inhibitors

Inhibitors Comment Organism Structure
p-bromophenacyl bromide abolishes the enzymatic activity of PLA2 without affecting its anti-tumor effect, suggesting the presence of pharmacological sites distinct from the catalytic site Macrovipera lebetina transmediterranea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13627
-
x * 13627, mass spectrometry. x * 13638, calculated from sequence Macrovipera lebetina transmediterranea
13638
-
x * 13627, mass spectrometry. x * 13638, calculated from sequence Macrovipera lebetina transmediterranea

Organism

Organism UniProt Comment Textmining
Macrovipera lebetina transmediterranea B5U6Z2
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by gel filtration and reverse-phase chromatography Macrovipera lebetina transmediterranea

Source Tissue

Source Tissue Comment Organism Textmining
venom seems to be the unique PLA2 in this venom Macrovipera lebetina transmediterranea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63.51
-
at 37°C, pH 7 Macrovipera lebetina transmediterranea

Subunits

Subunits Comment Organism
? x * 13627, mass spectrometry. x * 13638, calculated from sequence Macrovipera lebetina transmediterranea

Synonyms

Synonyms Comment Organism
acidic Asp49 phospholipase A2
-
Macrovipera lebetina transmediterranea
group II snake venom phospholipase A2
-
Macrovipera lebetina transmediterranea
svPLA2
-
Macrovipera lebetina transmediterranea

pI Value

Organism Comment pI Value Maximum pI Value
Macrovipera lebetina transmediterranea calculated from sequence
-
4.69

General Information

General Information Comment Organism
physiological function PLA2 inhibits efficiently tumor cells adhesion and migration. PLA2 is not cytotoxic up to 0.002 mM during 4 days on human cell lines IGR39 (melanoma) and HT1080 (fibrosarcoma). Completely abolishes cell adhesion of IGR39 cells to fibrinogen and fibronectin in a dose-dependent manner, while attachment to laminin-1 and vitronectin is only slightly affected. The enzyme has no effect on type I collagen, integrin-independent substratum and poly-L-lysine. PLA2 abolishes HT1080 cells migration towards fibronectin and fibrinogen in a dose-dependent manner. Anti-tumor effect of PLA2 is mediated by alpha5beta1 and alphav-containing integrins Macrovipera lebetina transmediterranea