Application | Comment | Organism |
---|---|---|
analysis | simple and inexpensive protein expression, refolding and purification system may be useful for site-directed mutagenesis experiments of sPLA2-IID which will advance the understanding of the structurefunction relationship and biological effects of the protein | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DNA-coding sequence subcloned into the vector pET3a, and expressed as inclusion bodies in Escherichia coli strain BL21(DE3)[pLysS] | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | loss of hydrolytic activity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | shows Ca2+-dependent hydrolytic activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by single-step cation-exchange chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63 | - |
in 20 mM HEPES buffer, 20 mM NaCl, 1 mM CaCl2, pH and temperature not specified in the publication | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, in 5 mM Tris-HCl buffer, pH 7.5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | unilamellar liposomes composed of a 9:1 M ratio of dioleoyl phosphatidylcholine and dioleoyl phosphatidylglycerol, release of fatty acid | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
secreted group IID phospholipase A2 | - |
Homo sapiens |
sPLA2-IID | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | is involved in the regulation of immune and inflammatory responses | Homo sapiens |