Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-bromophenacyl bromide | selectively and irreversibly modifies the His48 residue in the active site | Naja kaouthia | |
additional information | 4-bromophenacyl bromide does not modify the enzyme, no incorporation of the 4-bromophenacyl group into the enzyme | Naja haje |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14340 | - |
mass spectrometry | Naja haje |
15840 | - |
ethylpyridylation after total reduction of the enzyme, mass spectrometry | Naja haje |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Naja haje | - |
- |
- |
Naja kaouthia | P00596 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration and ammonium acetate precipitation | Naja haje |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Naja haje | - |
venom | - |
Naja kaouthia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | does not hydrolyze either fibrinogen or thrombin | Naja haje | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Group IB phospholipase A2 | - |
Naja haje |
phospholipase A2 | - |
Naja kaouthia |
PLA2 | - |
Naja haje |
PLA2 CM2 | - |
Naja kaouthia |
thrombin inhibitor from Naja haje | - |
Naja haje |
TI-Nh | - |
Naja haje |
General Information | Comment | Organism |
---|---|---|
physiological function | does not impair the adhesion of PC12 cells to plates. Is at least 2 orders of magnitude more cytotoxic than thrombin inhibitor from Naja haje | Naja kaouthia |
physiological function | is a mixed-type inhibitor of thrombin and effectively inhibits thrombin-induced platelet aggregation. Does not require a plasma cofactor, the inhibition is direct. Strongly prolongs the thrombin clotting time (25fold at 0.0000475 mM) with whole human blood plasma. Possesses relatively weak enzymatic activity and is nontoxic to PC12 cells at concentrations up to 0.015 mM, but evokes neurite outgrowth in these cells at a concentration of approximately 0.001 mM, does not impair the adhesion of PC12 cells to plates. At concentrations up to approximately 0.00005 mM, at which the thrombin-clotting time is substantially prolonged, the enzyme exerts no detectable effects on both the intrinsic and extrinsic pathways of the coagulation cascade, thus is a selective thrombin inhibitor. Is at least 2 orders of magnitude less cytotoxic than PLA2 CM2 from Naja kaouthia | Naja haje |