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Literature summary for 3.1.1.43 extracted from

  • Sugihara, A.; Shimada, Y.; Sugihara, S.; Nagao, T.; Watanabe, Y.; Tominaga, Y.
    A novel alpha-amino-acid esterase from Bacillus mycoides capable of forming peptides of DD- and DL-configurations (2001), J. Biochem., 130, 119-126.
    View publication on PubMed
Search for more literature for 3.1.1.43

Inhibitors

Inhibitors Comment Organism Structure
phenylmethanesulfonyl fluoride 1 mM, 99% inhibition Bacillus mycoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 4 * 39000, SDS-PAGE Bacillus mycoides
160000
-
 gel filtration Bacillus mycoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an alpha-aminoacid ester + H2O Bacillus mycoides
-
 an alpha-amino acid + an alcohol
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus mycoides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Toyopearl, electrofocusing, Disc electrophoresis Bacillus mycoides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2160
-
-
 Bacillus mycoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-D-phenyl methyl ester + L-leucine amide
-
 Bacillus mycoides acetyl-D-phenyl-L-leucine + methanol
-
 ?
acetyl-D-phenyl methyl ester + L-phenylalanine amide
-
 Bacillus mycoides acetyl-D-phenyl-L-phenylalanine + methanol
-
 ?
acetyl-D-phenylalanine methyl ester + H2O
-
 Bacillus mycoides acetyl-D-phenylalanine + methanol
-
 r
acetyl-L-phenylalanine methyl ester + H2O
-
 Bacillus mycoides acetyl-L-phenylalanine + methanol
-
 r
an alpha-aminoacid ester + H2O
-
 Bacillus mycoides an alpha-amino acid + an alcohol
-
 ?
an alpha-aminoacid ester + H2O
-
 Bacillus mycoides an alpha-amino acid + an alcohol
-
 r
D-aspartate dimethyl ester + H2O
-
 Bacillus mycoides D-aspartate + methanol
-
 r
D-methionine methyl ester + H2O
-
 Bacillus mycoides D-methionine + methanol
-
 r
D-phenylalanine amide + H2O
-
 Bacillus mycoides D-phenylalanine + NH3
-
 ?
D-phenylalanine methyl ester + H2O
-
 Bacillus mycoides D-phenylalanine + methanol
-
 r
D-tryptophan methyl ester + H2O
-
 Bacillus mycoides D-tryptophan + methanol
-
 r
D-tyrosine methyl ester + H2O
-
 Bacillus mycoides D-tyrosine + methanol
-
 r
L-aspartate dimethyl ester + H2O
-
 Bacillus mycoides L-aspartate + methanol
-
 ?
L-methionine methyl ester + H2O
-
 Bacillus mycoides L-methionine + methanol
-
 r
L-phenylalanine methyl ester + H2O
-
 Bacillus mycoides L-phenylalanine + methanol
-
 r
L-tryptophan methyl ester + H2O
-
 Bacillus mycoides L-tryptophan + methanol
-
 r
L-tyrosine methyl ester + H2O
-
 Bacillus mycoides L-tyrosine + methanol
-
 r
methyl acetate 1.1% of activity with D-phenylalanine methylester Bacillus mycoides acetate + methanol
-
 ?
methyl butyrate 3.6% of activity with D-phenylalanine methylester Bacillus mycoides butyrate + methanol
-
 ?
methyl caproate 2% of activity with D-phenylalanine methylester Bacillus mycoides caproate + methanol
-
 ?
methyl caprylate 1.1% of activity with D-phenylalanine methylester Bacillus mycoides caprylate + methanol
-
 ?
methyl propionate 2.7% of activity with D-phenylalanine methylester Bacillus mycoides propionate + methanol
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Bacillus mycoides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
47
-
-
 Bacillus mycoides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 40% of maximal activity at 10°C, approx. 5% of maximal activity at 65°C Bacillus mycoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6160
-
 phenylalanine methylester 25°C, pH 7.0 Bacillus mycoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 hydrolysis of phenylalanine methylester Bacillus mycoides

pH Range

pH Minimum pH Maximum Comment Organism
4 11 approx. 10% of maximal activity at pH 4.0 and pH 10.0, respectively Bacillus mycoides

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus mycoides isoelectric focusing
-
 7