Cloned (Comment) | Organism |
---|---|
expression of His-tagged AEH in Escherichia coli | Acetobacter pasteurianus |
Protein Variants | Comment | Organism |
---|---|---|
D338A | 0.07% of wild-type kcat | Acetobacter pasteurianus |
H370A | 0.07% of wild-type kcat | Acetobacter pasteurianus |
H610A | more than 69% of wild-type kcat, partially purified | Acetobacter pasteurianus |
R85A | 67% of wild-type kcat | Acetobacter pasteurianus |
S156A | 48% of wild-type kcat | Acetobacter pasteurianus |
S205A | no activity | Acetobacter pasteurianus |
Y206A | 44% of wild-type kcat | Acetobacter pasteurianus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-nitrophenyl-p'-guanidino benzoate | reversible inactivation | Acetobacter pasteurianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
cephalexin | 30°C, pH 7.0 | Acetobacter pasteurianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alpha-aminoacid ester + H2O | Acetobacter pasteurianus | - |
an alpha-amino acid + an alcohol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter pasteurianus | - |
ATCC 9325 | - |
Purification (Comment) | Organism |
---|---|
recombinant AEH, Ni2+-agarose, gel filtration | Acetobacter pasteurianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alpha-aminoacid ester + H2O | - |
Acetobacter pasteurianus | an alpha-amino acid + an alcohol | - |
? | |
an alpha-aminoacid ester + H2O | - |
Acetobacter pasteurianus | an alpha-amino acid + an alcohol | - |
r | |
cephalexin + H2O | - |
Acetobacter pasteurianus | D-phenylglycine + 7-aminodesacetoxycephalosporanic acid | - |
r | |
p-nitrophenyl-p'-guanidino benzoate + H2O | - |
Acetobacter pasteurianus | p-guanidino benzoate + p-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AEH | - |
Acetobacter pasteurianus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
274 | - |
cephalexin | 30°C, pH 7.0 | Acetobacter pasteurianus |