Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chlorocatechols | growth on chlorocatechols induce the trans-dienelactone hydrolase activity by 6fold | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 34% remaining activity after 5 min, 1 mM | Pseudomonas sp. | |
EDTA | 27% remaining activity after 5 min, 17% remaining activity after 60 min, 1 mM | Pseudomonas sp. | |
Hg2+ | 6% remaining activity after 5 min, 1 mM | Pseudomonas sp. | |
additional information | no inhibition by 4-chloromercuribenzoate | Pseudomonas sp. | |
o-phenanthroline | 55% remaining activity after 5 min, 18% remaining activity after 60 min, 1 mM | Pseudomonas sp. | |
Zn2+ | below 5% remaining activity after 5 min, 1 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
trans-dienelactone | pH 7.5, 25°C | Pseudomonas sp. | |
1.2 | - |
4-fluoromuconolactone | pH 7.5, 25°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE | Pseudomonas sp. |
85000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas sp. | antibiotic protoanemonin is no substrate | ? | - |
? | |
additional information | Pseudomonas sp. MT1 | antibiotic protoanemonin is no substrate | ? | - |
? | |
trans-dienelactone + H2O | Pseudomonas sp. | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | maleylacetate | - |
? | |
trans-dienelactone + H2O | Pseudomonas sp. MT1 | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | maleylacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
possesses only the trans-substrate isomer-specific enzyme form | - |
Pseudomonas sp. MT1 | - |
possesses only the trans-substrate isomer-specific enzyme form | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
190 | - |
purified enzyme, substrate 4-fluoromuconolactone | Pseudomonas sp. |
710 | - |
purified enzyme, substrate trans-dienelactone | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-fluoromuconolactone + H2O | - |
Pseudomonas sp. | maleylacetate + HF | - |
? | |
4-fluoromuconolactone + H2O | - |
Pseudomonas sp. MT1 | maleylacetate + HF | - |
? | |
additional information | antibiotic protoanemonin is a poor substrate | Pseudomonas sp. | ? | - |
? | |
additional information | antibiotic protoanemonin is no substrate | Pseudomonas sp. | ? | - |
? | |
additional information | antibiotic protoanemonin is a poor substrate | Pseudomonas sp. MT1 | ? | - |
? | |
additional information | antibiotic protoanemonin is no substrate | Pseudomonas sp. MT1 | ? | - |
? | |
trans-dienelactone + H2O | very low activity with the cis-isomer | Pseudomonas sp. | maleylacetate | - |
? | |
trans-dienelactone + H2O | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | Pseudomonas sp. | maleylacetate | - |
? | |
trans-dienelactone + H2O | very low activity with the cis-isomer | Pseudomonas sp. MT1 | maleylacetate | - |
? | |
trans-dienelactone + H2O | degradation of chlorosalicylates via chlorocatechols to 3-oxoadipate via maleylacetate, pathway overview | Pseudomonas sp. MT1 | maleylacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
cis-dienelactone hydrolase | - |
Pseudomonas sp. |
cis-DLH | - |
Pseudomonas sp. |
dienelactone hydrolase | - |
Pseudomonas sp. |
DLH | - |
Pseudomonas sp. |
trans-dienelactone hydrolase | - |
Pseudomonas sp. |
trans-DLH | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1630 | - |
4-fluoromuconolactone | pH 7.5, 25°C | Pseudomonas sp. | |
1633 | - |
4-fluoromuconolactone | pH 7.5, 25°C | Pseudomonas sp. | |
2650 | - |
trans-dienelactone | pH 7.5, 25°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas sp. |