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Literature summary for 3.1.1.45 extracted from
- Dienelactone hydrolase from Pseudomonas sp. strain B13 (1987), J. Bacteriol., 169, 699-703.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement | Pseudomonas knackmussii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas knackmussii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | inactivation completely reversed by dithiothreitol | Pseudomonas knackmussii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
trans-4-Carboxymethylenebut-2-en-4-olide | - |
Pseudomonas knackmussii | |
| 0.4 | - |
cis-4-carboxymethylenebut-2-en-4-olide | - |
Pseudomonas knackmussii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Pseudomonas knackmussii | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement | Pseudomonas knackmussii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28500 | - |
gel filtration and nondenaturing PAGE | Pseudomonas knackmussii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxymethylenebut-2-en-4-olide + H2O | Pseudomonas knackmussii | dienelactone hydrolase catalyzes a step in the metabolic conversion of chlorocatechols to beta-ketoadipate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas knackmussii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3-chlorobenzoate-grown cells | Pseudomonas knackmussii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Pseudomonas knackmussii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM Tris-HCl, pH 7.4, 10 micromol dithiothreitol, stable for over 6 months | Pseudomonas knackmussii |
| 4°C, converted to microcrystals, ammonium sulfate to 30% saturation, stable for more than 1 year | Pseudomonas knackmussii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxymethylenebut-2-en-4-olide + H2O | dienelactone hydrolase catalyzes a step in the metabolic conversion of chlorocatechols to beta-ketoadipate | Pseudomonas knackmussii | ? | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1* 28000, SDS-PAGE with mercaptoethanol | Pseudomonas knackmussii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
4-carboxymethylenebut-2-en-4-olide | - |
Pseudomonas knackmussii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas knackmussii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Pseudomonas knackmussii |
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