Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Origami B cells | Tannerella forsythia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0512 | - |
4-nitrophenyl acetate | at pH 7.4 and 25°C | Tannerella forsythia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tannerella forsythia | G8UJ84 | - |
- |
Tannerella forsythia 92A.2 | G8UJ84 | - |
- |
Purification (Comment) | Organism |
---|---|
GSTrap column chromatography, and Superdex 200 gel filtration | Tannerella forsythia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Tannerella forsythia | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Tannerella forsythia 92A.2 | 4-nitrophenol + acetate | - |
? | |
additional information | the enzyme show no activity with N-acetyl-O-5,4-diacetylneuraminate | Tannerella forsythia | ? | - |
? | |
additional information | the enzyme show no activity with N-acetyl-O-5,4-diacetylneuraminate | Tannerella forsythia 92A.2 | ? | - |
? | |
N-acetyl-O-5,9-diacetylneuraminate + H2O | - |
Tannerella forsythia | N-acetylneuraminate + acetate | - |
? | |
N-acetyl-O-5,9-diacetylneuraminate + H2O | - |
Tannerella forsythia 92A.2 | N-acetylneuraminate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76300, mature protein, calculated from amino acid sequence | Tannerella forsythia |
Synonyms | Comment | Organism |
---|---|---|
NANS | - |
Tannerella forsythia |
sialate-O-acetylesterase | - |
Tannerella forsythia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
111 | - |
4-nitrophenyl acetate | at pH 7.4 and 25°C | Tannerella forsythia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2200 | - |
4-nitrophenyl acetate | at pH 7.4 and 25°C | Tannerella forsythia |