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Literature summary for 3.1.1.61 extracted from
- Purification and characterization of chemotactic methylesterase from Bacillus subtilis (1984), Biochemistry, 23, 675-680.
General Stability
| General Stability | Organism |
|---|---|
| stabilized by aqueous glycerol solution | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00001 | - |
methylated methyl-accepting chemotaxis protein | pH 7.0, 22°C | Bacillus subtilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bacillus subtilis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required | Bacillus subtilis |  |
| Mg2+ | maximal activity with 1.1 mM Mg2+ | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 1 month | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl-accepting chemotaxis protein + H2O | - |
Bacillus subtilis | chemotaxis protein + methanol | - |
? | |
| methylated methyl-accepting chemotaxis protein + H2O | - |
Bacillus subtilis | methyl-accepting chemotaxis protein + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41000 SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 37 | 50% of activity maximum at 20°C and 37°C, about 60% of activity maximum at 22°C | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
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