Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
expression in Escherichia coli | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
additional information | constructs of the N- and C-terminal domains and investigation of interaction between the isolated regulatory and effector domains of enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
additional information | constructs of the N- and C-terminal domains and investigation of interaction between the isolated regulatory and effector domains of enzyme | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the unphosphorylated regulator domain partially inhibits methylesterase | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | the unphosphorylated regulator domain partially inhibits methylesterase | Thermotoga maritima | |
P2 domain of histidine kinase | inhibition is decreased upon phosphorylation | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | In its unphosphorylated state, the regulatory domain inhibits enzyme activity of effector domain. Phosphorylation of the regulator domain leads to an enhancement of enzyme activity through a relief of inhibition and a stimulatory effect on catalysis. | Salmonella enterica subsp. enterica serovar Typhimurium |
phosphoprotein | In its unphosphorylated state, the regulatory domain inhibits enzyme activity of effector domain. Phosphorylation of the regulator domain leads to an enhancement of enzyme activity through a relief of inhibition and a stimulatory effect on catalysis. | Thermotoga maritima |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
recombinant enzyme | Thermotoga maritima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol | the enzyme is a response regulator protein in the bacterial chemotaxis two-component signal transduction pathway | Salmonella enterica subsp. enterica serovar Typhimurium |
Subunits | Comment | Organism |
---|---|---|
More | the CheB protein is composed of at least 2 structurally distinct proportions: a C-terminal catalytic domain, and a N-terminal region which modulates esterase activity | Salmonella enterica subsp. enterica serovar Typhimurium |
More | the CheB protein is composed of at least 2 structurally distinct proportions: a C-terminal catalytic domain, and a N-terminal region which modulates esterase activity | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
P2 domain of histidine kinase | pH 7.0, 25°C, in absence of phosphoramidate | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.021 | - |
P2 domain of histidine kinase | pH 7.0, 25°C, in presence of phosphoramidate | Salmonella enterica subsp. enterica serovar Typhimurium |